Normal mammary epithelial cells promote carcinoma basement membrane invasion by inducing microtubule-rich protrusions

Meng Horng Lee, Pei Hsun Wu, Daniele Gilkes, Ivie Aifuwa, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Recent work suggests that the dissemination of tumor cells may occur in parallel with, and even preceed, tumor growth. The mechanism for this early invasion is largely unknown. Here, we find that mammary epithelial cells (MECs) induce neighboring breast carcinoma cells (BCCs) to cross the basement membrane by secreting soluble laminin. Laminin continuously produced by MECs induce long membrane cellular protrusions in BCCs that promote their contractility and invasion into the surrounding matrix. These protrusions depend on microtubule bundles assembled de novo through laminin-integrin ß1 signaling. These results describe how non-cancerous MECs can actively participate in the invasive process of BCCs.

Original languageEnglish (US)
Pages (from-to)32634-32645
Number of pages12
JournalOncotarget
Volume6
Issue number32
DOIs
StatePublished - 2015

Fingerprint

Basement Membrane
Microtubules
Breast
Epithelial Cells
Carcinoma
Laminin
Breast Neoplasms
Cell Surface Extensions
Integrins
Neoplasms
Membranes
Growth

Keywords

  • Cancer invasion
  • Cell-cell interaction
  • Laminin
  • Microtubule
  • Protrusion

ASJC Scopus subject areas

  • Oncology

Cite this

Normal mammary epithelial cells promote carcinoma basement membrane invasion by inducing microtubule-rich protrusions. / Lee, Meng Horng; Wu, Pei Hsun; Gilkes, Daniele; Aifuwa, Ivie; Wirtz, Denis.

In: Oncotarget, Vol. 6, No. 32, 2015, p. 32634-32645.

Research output: Contribution to journalArticle

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