Normal and deficient neutrophils can cooperate to damage aspergillus fumigatus hyphae

John H. Rex, John E. Bennett, John I. Gallin, Harry L. Malech, David A. Melnick

Research output: Contribution to journalArticlepeer-review

Abstract

Using a metabolic test of hyphal viability, the interaction between neutrophils and Aspergillus hyphae was investigated over a broad range of hyphae-to-neutrophil ratios. Normal neutrophils were found to damage hyphae whereas neutrophils from patients with both chronic granulomatous disease (CGD) and myeloperoxidase (MPO) deficiency did not. Further, both azide and catalase + superoxide dismutase inhibited the ability of normal neutrophils to damage hyphae, suggesting that this damage is mediated by products of the respiratory burst and by the MPO-halide system. Also, mixtures of small numbers of normal neutrophils with larger numbers of CGD neutrophils (range, 1:5 to 1:15) damaged hyphae more efficiently than either population of cells alone. Further, mixtures ofCGD and MPO-deficient neutrophils, neither of which alone could efficiently damage hyphae, were able to damage the hyphae almost as well as a comparable number of normal neutrophils. These data demonstrate that intact neutrophils can cooperate to synergistically damage Aspergillus hyphae, possibly by extracellular mixing of hydrogen peroxide and MPO.

Original languageEnglish (US)
Pages (from-to)523-528
Number of pages6
JournalJournal of Infectious Diseases
Volume162
Issue number2
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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