Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Wenqian Hu; Christine Petzold; Jeff Coller; Kristian E. Baker

doi:10.1038/nsmb.1734

Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Wenqian Hu, Christine Petzold, Jeff Coller, Kristian E. Baker

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

Original language	English (US)
Pages (from-to)	244-247
Number of pages	4
Journal	Nature Structural and Molecular Biology
Volume	17
Issue number	2
DOIs	https://doi.org/10.1038/nsmb.1734
State	Published - Feb 2010
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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title = "Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae",

abstract = "Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.",

author = "Wenqian Hu and Christine Petzold and Jeff Coller and Baker, {Kristian E.}",

note = "Funding Information: The authors thank members of the Baker and Coller laboratories for critical evaluation of the manuscript. W.H. is supported by the American Heart Association. C.P. is supported by an undergraduate stipend provided by the US National Science Foundation. Funding was provided by the US National Institutes of Health (GM080465).",

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N1 - Funding Information: The authors thank members of the Baker and Coller laboratories for critical evaluation of the manuscript. W.H. is supported by the American Heart Association. C.P. is supported by an undergraduate stipend provided by the US National Science Foundation. Funding was provided by the US National Institutes of Health (GM080465).

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AB - Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

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Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Abstract

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