Noninvasive visualization of the choriocapillaris and its dynamic filling

J. Kiryu, M. Shahidi, M. T. Mori, Y. Ogura, S. Asrani, Ran Zeimer

Research output: Contribution to journalArticle

Abstract

Purpose. The choroidal microvasculature and its circulation are inadequately assessed by presently available techniques. Laser-targeted delivery was applied to generate local, repetitive angiograms of the choriocapillaris in primates. Methods. Carboxyfluorescein was encapsulated in heat-sensitive liposomes and injected intravenously in monkeys. The liposome contents were then released locally in the choroid by application of a short heat pulse provided by an infrared laser. The bolus of dye spread rapidly downstream from the underlying arterioles into clusters of lobules. Video angiograms were generated with excitation illumination provided by an argon laser. Results. Laser-targeted delivery choroidal angiography performed on three monkeys indicated that the fluorescence was emitted mainly from the choriocapillaris. Clusters of irregular shape with well-defined margins were observed. Adjacent arteries typically supplied separate clusters that fit together like a jigsaw puzzle. The dynamic filling and emptying patterns, recorded at video rate, revealed that macular lobules were filled by a central arteriole and drained by a venous annulus. The average dye transit time through a lobule (n = 10) was 118 ± 26 msec (mean ± SD), and the dye transit velocity was 2.53 ± 0.55 mm/sec. Conclusions. This study clearly documents the segmental nature of the primate choroidal microvasculature. It also illustrates that choroidal angiography by laser-targeted dye delivery provides information useful for studying the response of the choriocapillaris to physiological and pathologic changes.

Original languageEnglish (US)
Pages (from-to)3724-3731
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume35
Issue number10
StatePublished - 1994

Fingerprint

Angiography
Lasers
Coloring Agents
Arterioles
Microvessels
Liposomes
Primates
Haplorhini
Hot Temperature
Dye Lasers
Choroid
Argon
Lighting
Arteries
Fluorescence

Keywords

  • angiography
  • choriocapillaris
  • drug delivery
  • hemodynamics
  • liposomes

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kiryu, J., Shahidi, M., Mori, M. T., Ogura, Y., Asrani, S., & Zeimer, R. (1994). Noninvasive visualization of the choriocapillaris and its dynamic filling. Investigative Ophthalmology and Visual Science, 35(10), 3724-3731.

Noninvasive visualization of the choriocapillaris and its dynamic filling. / Kiryu, J.; Shahidi, M.; Mori, M. T.; Ogura, Y.; Asrani, S.; Zeimer, Ran.

In: Investigative Ophthalmology and Visual Science, Vol. 35, No. 10, 1994, p. 3724-3731.

Research output: Contribution to journalArticle

Kiryu, J, Shahidi, M, Mori, MT, Ogura, Y, Asrani, S & Zeimer, R 1994, 'Noninvasive visualization of the choriocapillaris and its dynamic filling', Investigative Ophthalmology and Visual Science, vol. 35, no. 10, pp. 3724-3731.
Kiryu, J. ; Shahidi, M. ; Mori, M. T. ; Ogura, Y. ; Asrani, S. ; Zeimer, Ran. / Noninvasive visualization of the choriocapillaris and its dynamic filling. In: Investigative Ophthalmology and Visual Science. 1994 ; Vol. 35, No. 10. pp. 3724-3731.
@article{957022000277478e97edeca0fd3a9d5e,
title = "Noninvasive visualization of the choriocapillaris and its dynamic filling",
abstract = "Purpose. The choroidal microvasculature and its circulation are inadequately assessed by presently available techniques. Laser-targeted delivery was applied to generate local, repetitive angiograms of the choriocapillaris in primates. Methods. Carboxyfluorescein was encapsulated in heat-sensitive liposomes and injected intravenously in monkeys. The liposome contents were then released locally in the choroid by application of a short heat pulse provided by an infrared laser. The bolus of dye spread rapidly downstream from the underlying arterioles into clusters of lobules. Video angiograms were generated with excitation illumination provided by an argon laser. Results. Laser-targeted delivery choroidal angiography performed on three monkeys indicated that the fluorescence was emitted mainly from the choriocapillaris. Clusters of irregular shape with well-defined margins were observed. Adjacent arteries typically supplied separate clusters that fit together like a jigsaw puzzle. The dynamic filling and emptying patterns, recorded at video rate, revealed that macular lobules were filled by a central arteriole and drained by a venous annulus. The average dye transit time through a lobule (n = 10) was 118 ± 26 msec (mean ± SD), and the dye transit velocity was 2.53 ± 0.55 mm/sec. Conclusions. This study clearly documents the segmental nature of the primate choroidal microvasculature. It also illustrates that choroidal angiography by laser-targeted dye delivery provides information useful for studying the response of the choriocapillaris to physiological and pathologic changes.",
keywords = "angiography, choriocapillaris, drug delivery, hemodynamics, liposomes",
author = "J. Kiryu and M. Shahidi and Mori, {M. T.} and Y. Ogura and S. Asrani and Ran Zeimer",
year = "1994",
language = "English (US)",
volume = "35",
pages = "3724--3731",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "10",

}

TY - JOUR

T1 - Noninvasive visualization of the choriocapillaris and its dynamic filling

AU - Kiryu, J.

AU - Shahidi, M.

AU - Mori, M. T.

AU - Ogura, Y.

AU - Asrani, S.

AU - Zeimer, Ran

PY - 1994

Y1 - 1994

N2 - Purpose. The choroidal microvasculature and its circulation are inadequately assessed by presently available techniques. Laser-targeted delivery was applied to generate local, repetitive angiograms of the choriocapillaris in primates. Methods. Carboxyfluorescein was encapsulated in heat-sensitive liposomes and injected intravenously in monkeys. The liposome contents were then released locally in the choroid by application of a short heat pulse provided by an infrared laser. The bolus of dye spread rapidly downstream from the underlying arterioles into clusters of lobules. Video angiograms were generated with excitation illumination provided by an argon laser. Results. Laser-targeted delivery choroidal angiography performed on three monkeys indicated that the fluorescence was emitted mainly from the choriocapillaris. Clusters of irregular shape with well-defined margins were observed. Adjacent arteries typically supplied separate clusters that fit together like a jigsaw puzzle. The dynamic filling and emptying patterns, recorded at video rate, revealed that macular lobules were filled by a central arteriole and drained by a venous annulus. The average dye transit time through a lobule (n = 10) was 118 ± 26 msec (mean ± SD), and the dye transit velocity was 2.53 ± 0.55 mm/sec. Conclusions. This study clearly documents the segmental nature of the primate choroidal microvasculature. It also illustrates that choroidal angiography by laser-targeted dye delivery provides information useful for studying the response of the choriocapillaris to physiological and pathologic changes.

AB - Purpose. The choroidal microvasculature and its circulation are inadequately assessed by presently available techniques. Laser-targeted delivery was applied to generate local, repetitive angiograms of the choriocapillaris in primates. Methods. Carboxyfluorescein was encapsulated in heat-sensitive liposomes and injected intravenously in monkeys. The liposome contents were then released locally in the choroid by application of a short heat pulse provided by an infrared laser. The bolus of dye spread rapidly downstream from the underlying arterioles into clusters of lobules. Video angiograms were generated with excitation illumination provided by an argon laser. Results. Laser-targeted delivery choroidal angiography performed on three monkeys indicated that the fluorescence was emitted mainly from the choriocapillaris. Clusters of irregular shape with well-defined margins were observed. Adjacent arteries typically supplied separate clusters that fit together like a jigsaw puzzle. The dynamic filling and emptying patterns, recorded at video rate, revealed that macular lobules were filled by a central arteriole and drained by a venous annulus. The average dye transit time through a lobule (n = 10) was 118 ± 26 msec (mean ± SD), and the dye transit velocity was 2.53 ± 0.55 mm/sec. Conclusions. This study clearly documents the segmental nature of the primate choroidal microvasculature. It also illustrates that choroidal angiography by laser-targeted dye delivery provides information useful for studying the response of the choriocapillaris to physiological and pathologic changes.

KW - angiography

KW - choriocapillaris

KW - drug delivery

KW - hemodynamics

KW - liposomes

UR - http://www.scopus.com/inward/record.url?scp=0027970758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027970758&partnerID=8YFLogxK

M3 - Article

C2 - 8088959

AN - SCOPUS:0027970758

VL - 35

SP - 3724

EP - 3731

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 10

ER -