Noninvasive tracking of alginate-microencapsulated cells

Genaro A. Paredes-Juarez, Brad P. Barnett, Jeff W Bulte

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter presents a description of standardized techniques used routinely in our laboratory to encapsulate different cell types using the alginate-PLL-alginate immunoisolation system. Given the importance of noninvasive tracking of encapsulated cell transplants, we present a detailed guidance to achieve maximum efficiency and functionality of the capsule preparations for optimal tracking posttransplantation. The provided protocols cover tracking of encapsulated cells using magnetic resonance (MR), X-ray, computed tomography (CT), and ultrasound (US) imaging. Practical suggestions to optimize each method with specific references to recommended suppliers are included.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-155
Number of pages13
Volume1479
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1479
ISSN (Print)10643745

Keywords

  • Alginate
  • Encapsulation
  • MRI
  • Noninvasive tracking
  • Ultrasound
  • X-ray

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Noninvasive tracking of alginate-microencapsulated cells'. Together they form a unique fingerprint.

  • Cite this

    Paredes-Juarez, G. A., Barnett, B. P., & Bulte, J. W. (2017). Noninvasive tracking of alginate-microencapsulated cells. In Methods in Molecular Biology (Vol. 1479, pp. 143-155). (Methods in Molecular Biology; Vol. 1479). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6364-5_11