Noninvasive tracking of alginate-microencapsulated cells

Genaro A. Paredes-Juarez, Brad P. Barnett, Jeff W.M. Bulte

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This chapter presents a description of standardized techniques used routinely in our laboratory to encapsulate different cell types using the alginate-PLL-alginate immunoisolation system. Given the importance of noninvasive tracking of encapsulated cell transplants, we present a detailed guidance to achieve maximum efficiency and functionality of the capsule preparations for optimal tracking posttransplantation. The provided protocols cover tracking of encapsulated cells using magnetic resonance (MR), X-ray, computed tomography (CT), and ultrasound (US) imaging. Practical suggestions to optimize each method with specific references to recommended suppliers are included.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages143-155
Number of pages13
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1479
ISSN (Print)1064-3745

Keywords

  • Alginate
  • Encapsulation
  • MRI
  • Noninvasive tracking
  • Ultrasound
  • X-ray

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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