Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging: A proof of concept study

Julius Renne, Jan Hinrichs, Christian Schönfeld, Marcel Gutberlet, Carla Winkler, Cornelia Faulenbach, Peter Jakob, Frank Schaumann, Norbert Krug, Frank Wacker, Jens M. Hohlfeld, Jens Vogel-Claussen

Research output: Contribution to journalArticle

Abstract

Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.

Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours after segmental allergen challenge by using either normal-or low-dose allergen or saline. The volume of lung tissue with increased relaxation times was determined by using a threshold-based method. As a biomarker for oxygen transfer from the lungs into the blood, the oxygen transfer function (OTF) was calculated. After the third MR imaging examination, eosinophils in bronchoalveolar lavage fluid were counted. Differences between times and segments were analyzed with nonparametric Wilcoxon matched-pairs test and Spearman correlation.

Results: In lung segments treated with the standard dose of allergen, the OTF was decreased at 6 hours in asthmatic patients, compared with saline-treated segments (P = .0078). In asthmatic patients at 24 hours, the volume over threshold was significantly increased in normal allergen dose-treated segments compared with saline-treated segments (P = .004). In corresponding lung segments, the volume over threshold at 24 hours in the asthmatic group showed a positive correlation (r = 0.65, P = .0001) and the OTF at 6 hours showed an inverse correlation (r = 20.67, P = .0001) with the percentage of eosinophils in the bron-choalveolar lavage fluid.

Conclusion: OTF and volume over threshold are noninvasive MR im-aging-derived parameters to visualize and quantify the regional allergic reaction after segmental endobronchial allergen challenge.

Original languageEnglish (US)
Pages (from-to)267-275
Number of pages9
JournalRadiology
Volume274
Issue number1
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Allergens
Magnetic Resonance Imaging
Oxygen
Inflammation
Lung
Eosinophils
Bronchial Provocation Tests
Research Ethics Committees
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Informed Consent
Hypersensitivity
Magnetic Resonance Spectroscopy
Biomarkers

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Medicine(all)

Cite this

Renne, J., Hinrichs, J., Schönfeld, C., Gutberlet, M., Winkler, C., Faulenbach, C., ... Vogel-Claussen, J. (2015). Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging: A proof of concept study. Radiology, 274(1), 267-275. https://doi.org/10.1148/radiol.14132607

Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging : A proof of concept study. / Renne, Julius; Hinrichs, Jan; Schönfeld, Christian; Gutberlet, Marcel; Winkler, Carla; Faulenbach, Cornelia; Jakob, Peter; Schaumann, Frank; Krug, Norbert; Wacker, Frank; Hohlfeld, Jens M.; Vogel-Claussen, Jens.

In: Radiology, Vol. 274, No. 1, 01.01.2015, p. 267-275.

Research output: Contribution to journalArticle

Renne, J, Hinrichs, J, Schönfeld, C, Gutberlet, M, Winkler, C, Faulenbach, C, Jakob, P, Schaumann, F, Krug, N, Wacker, F, Hohlfeld, JM & Vogel-Claussen, J 2015, 'Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging: A proof of concept study', Radiology, vol. 274, no. 1, pp. 267-275. https://doi.org/10.1148/radiol.14132607
Renne, Julius ; Hinrichs, Jan ; Schönfeld, Christian ; Gutberlet, Marcel ; Winkler, Carla ; Faulenbach, Cornelia ; Jakob, Peter ; Schaumann, Frank ; Krug, Norbert ; Wacker, Frank ; Hohlfeld, Jens M. ; Vogel-Claussen, Jens. / Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging : A proof of concept study. In: Radiology. 2015 ; Vol. 274, No. 1. pp. 267-275.
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abstract = "Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours after segmental allergen challenge by using either normal-or low-dose allergen or saline. The volume of lung tissue with increased relaxation times was determined by using a threshold-based method. As a biomarker for oxygen transfer from the lungs into the blood, the oxygen transfer function (OTF) was calculated. After the third MR imaging examination, eosinophils in bronchoalveolar lavage fluid were counted. Differences between times and segments were analyzed with nonparametric Wilcoxon matched-pairs test and Spearman correlation.Results: In lung segments treated with the standard dose of allergen, the OTF was decreased at 6 hours in asthmatic patients, compared with saline-treated segments (P = .0078). In asthmatic patients at 24 hours, the volume over threshold was significantly increased in normal allergen dose-treated segments compared with saline-treated segments (P = .004). In corresponding lung segments, the volume over threshold at 24 hours in the asthmatic group showed a positive correlation (r = 0.65, P = .0001) and the OTF at 6 hours showed an inverse correlation (r = 20.67, P = .0001) with the percentage of eosinophils in the bron-choalveolar lavage fluid.Conclusion: OTF and volume over threshold are noninvasive MR im-aging-derived parameters to visualize and quantify the regional allergic reaction after segmental endobronchial allergen challenge.",
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T1 - Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging

T2 - A proof of concept study

AU - Renne, Julius

AU - Hinrichs, Jan

AU - Schönfeld, Christian

AU - Gutberlet, Marcel

AU - Winkler, Carla

AU - Faulenbach, Cornelia

AU - Jakob, Peter

AU - Schaumann, Frank

AU - Krug, Norbert

AU - Wacker, Frank

AU - Hohlfeld, Jens M.

AU - Vogel-Claussen, Jens

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N2 - Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours after segmental allergen challenge by using either normal-or low-dose allergen or saline. The volume of lung tissue with increased relaxation times was determined by using a threshold-based method. As a biomarker for oxygen transfer from the lungs into the blood, the oxygen transfer function (OTF) was calculated. After the third MR imaging examination, eosinophils in bronchoalveolar lavage fluid were counted. Differences between times and segments were analyzed with nonparametric Wilcoxon matched-pairs test and Spearman correlation.Results: In lung segments treated with the standard dose of allergen, the OTF was decreased at 6 hours in asthmatic patients, compared with saline-treated segments (P = .0078). In asthmatic patients at 24 hours, the volume over threshold was significantly increased in normal allergen dose-treated segments compared with saline-treated segments (P = .004). In corresponding lung segments, the volume over threshold at 24 hours in the asthmatic group showed a positive correlation (r = 0.65, P = .0001) and the OTF at 6 hours showed an inverse correlation (r = 20.67, P = .0001) with the percentage of eosinophils in the bron-choalveolar lavage fluid.Conclusion: OTF and volume over threshold are noninvasive MR im-aging-derived parameters to visualize and quantify the regional allergic reaction after segmental endobronchial allergen challenge.

AB - Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours after segmental allergen challenge by using either normal-or low-dose allergen or saline. The volume of lung tissue with increased relaxation times was determined by using a threshold-based method. As a biomarker for oxygen transfer from the lungs into the blood, the oxygen transfer function (OTF) was calculated. After the third MR imaging examination, eosinophils in bronchoalveolar lavage fluid were counted. Differences between times and segments were analyzed with nonparametric Wilcoxon matched-pairs test and Spearman correlation.Results: In lung segments treated with the standard dose of allergen, the OTF was decreased at 6 hours in asthmatic patients, compared with saline-treated segments (P = .0078). In asthmatic patients at 24 hours, the volume over threshold was significantly increased in normal allergen dose-treated segments compared with saline-treated segments (P = .004). In corresponding lung segments, the volume over threshold at 24 hours in the asthmatic group showed a positive correlation (r = 0.65, P = .0001) and the OTF at 6 hours showed an inverse correlation (r = 20.67, P = .0001) with the percentage of eosinophils in the bron-choalveolar lavage fluid.Conclusion: OTF and volume over threshold are noninvasive MR im-aging-derived parameters to visualize and quantify the regional allergic reaction after segmental endobronchial allergen challenge.

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