Noninvasive localized MR quantification of creatine kinase metabolites in normal and infarcted canine myocardium

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Abstract

PURPOSE: To develop image-guided spatially localized magnetic resonance (MR) spectroscopy to provide a noninvasive quantitative probe of myocardial creatine kinase (CK) metabolism, and to use it to determine the extent of changes in CK energy metabolism in nonviable infarcted canine myocardium. MATERIALS AND METHODS: Water-referenced localized phosphorus and proton MR spectroscopy were combined in a single protocol to noninvasively measure phosphocreatine (PCr), adenosine triphosphate (ATP), and total of phosphorylated and unphosphorylated creatine (CR) concentrations and pH in the myocardium in six normal dogs and six dogs with surgically induced myocardial infarction. Unphosphorylated creatine and adenosine diphosphate (ADP) levels were calculated. The results were compared with biochemical measurements at postmortem biopsy. RESULTS: Significant reductions in PCr-to-ATP ratios (1.7 ± 0.3 [SD] vs 1 ± 0.4; P <.001), PCr (10.3 ± 2.1 vs 4.3 ± 2.0 μmol/g wet weight; P <.0001), ATP (6.4 ± 1.4 vs 3.7 ± 1.4 μmol/g wet weight; P <.001), and CR (24.7 ± 6.1 vs 6.3 ± 3.7; P <.0001) were measured noninvasively in infarcted, as compared with normal, tissue. Biopsy measurements confirmed infarct-related reductions observed at MR spectroscopy, although high-energy phosphate concentrations were lower at biopsy. ADP calculated from noninvasive MR spectroscopic measurements was 0.11 ± 0.07 μmol/g wet weight in normal myocardium. CONCLUSION: This combined phosphorus and proton MR spectroscopic approach provides a near-complete picture of in vivo myocardial CK metabolism in normal and diseased heart and a tool for noninvasively measuring metabolite reductions associated with the loss of viability.

Original languageEnglish (US)
Pages (from-to)411-418
Number of pages8
JournalRadiology
Volume219
Issue number2
StatePublished - 2001

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Creatine Kinase
Phosphocreatine
Creatine
Canidae
Myocardium
Magnetic Resonance Spectroscopy
MB Form Creatine Kinase
Adenosine Triphosphate
Biopsy
Weights and Measures
Adenosine Diphosphate
Phosphorus
Dogs
Energy Metabolism
Protons
Heart Diseases
Phosphates
Myocardial Infarction
Water

Keywords

  • Animals Heart, MR, 511.121411, 511.121415
  • Heart, MR spectroscopy, 511.12145
  • Magnetic resonance (MR), Phosphorus studies, 511.121411, 511.121415, 511.12147
  • Magnetic resonance (MR), spectroscopy, 511.12145
  • Metabolism, 511.91
  • Myocardium, infarction, 511.814

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

@article{919a6dbd826642348b45f71e1e1952ef,
title = "Noninvasive localized MR quantification of creatine kinase metabolites in normal and infarcted canine myocardium",
abstract = "PURPOSE: To develop image-guided spatially localized magnetic resonance (MR) spectroscopy to provide a noninvasive quantitative probe of myocardial creatine kinase (CK) metabolism, and to use it to determine the extent of changes in CK energy metabolism in nonviable infarcted canine myocardium. MATERIALS AND METHODS: Water-referenced localized phosphorus and proton MR spectroscopy were combined in a single protocol to noninvasively measure phosphocreatine (PCr), adenosine triphosphate (ATP), and total of phosphorylated and unphosphorylated creatine (CR) concentrations and pH in the myocardium in six normal dogs and six dogs with surgically induced myocardial infarction. Unphosphorylated creatine and adenosine diphosphate (ADP) levels were calculated. The results were compared with biochemical measurements at postmortem biopsy. RESULTS: Significant reductions in PCr-to-ATP ratios (1.7 ± 0.3 [SD] vs 1 ± 0.4; P <.001), PCr (10.3 ± 2.1 vs 4.3 ± 2.0 μmol/g wet weight; P <.0001), ATP (6.4 ± 1.4 vs 3.7 ± 1.4 μmol/g wet weight; P <.001), and CR (24.7 ± 6.1 vs 6.3 ± 3.7; P <.0001) were measured noninvasively in infarcted, as compared with normal, tissue. Biopsy measurements confirmed infarct-related reductions observed at MR spectroscopy, although high-energy phosphate concentrations were lower at biopsy. ADP calculated from noninvasive MR spectroscopic measurements was 0.11 ± 0.07 μmol/g wet weight in normal myocardium. CONCLUSION: This combined phosphorus and proton MR spectroscopic approach provides a near-complete picture of in vivo myocardial CK metabolism in normal and diseased heart and a tool for noninvasively measuring metabolite reductions associated with the loss of viability.",
keywords = "Animals Heart, MR, 511.121411, 511.121415, Heart, MR spectroscopy, 511.12145, Magnetic resonance (MR), Phosphorus studies, 511.121411, 511.121415, 511.12147, Magnetic resonance (MR), spectroscopy, 511.12145, Metabolism, 511.91, Myocardium, infarction, 511.814",
author = "Bottomley, {Paul A} and Weiss, {Robert George}",
year = "2001",
language = "English (US)",
volume = "219",
pages = "411--418",
journal = "Radiology",
issn = "0033-8419",
publisher = "Radiological Society of North America Inc.",
number = "2",

}

TY - JOUR

T1 - Noninvasive localized MR quantification of creatine kinase metabolites in normal and infarcted canine myocardium

AU - Bottomley, Paul A

AU - Weiss, Robert George

PY - 2001

Y1 - 2001

N2 - PURPOSE: To develop image-guided spatially localized magnetic resonance (MR) spectroscopy to provide a noninvasive quantitative probe of myocardial creatine kinase (CK) metabolism, and to use it to determine the extent of changes in CK energy metabolism in nonviable infarcted canine myocardium. MATERIALS AND METHODS: Water-referenced localized phosphorus and proton MR spectroscopy were combined in a single protocol to noninvasively measure phosphocreatine (PCr), adenosine triphosphate (ATP), and total of phosphorylated and unphosphorylated creatine (CR) concentrations and pH in the myocardium in six normal dogs and six dogs with surgically induced myocardial infarction. Unphosphorylated creatine and adenosine diphosphate (ADP) levels were calculated. The results were compared with biochemical measurements at postmortem biopsy. RESULTS: Significant reductions in PCr-to-ATP ratios (1.7 ± 0.3 [SD] vs 1 ± 0.4; P <.001), PCr (10.3 ± 2.1 vs 4.3 ± 2.0 μmol/g wet weight; P <.0001), ATP (6.4 ± 1.4 vs 3.7 ± 1.4 μmol/g wet weight; P <.001), and CR (24.7 ± 6.1 vs 6.3 ± 3.7; P <.0001) were measured noninvasively in infarcted, as compared with normal, tissue. Biopsy measurements confirmed infarct-related reductions observed at MR spectroscopy, although high-energy phosphate concentrations were lower at biopsy. ADP calculated from noninvasive MR spectroscopic measurements was 0.11 ± 0.07 μmol/g wet weight in normal myocardium. CONCLUSION: This combined phosphorus and proton MR spectroscopic approach provides a near-complete picture of in vivo myocardial CK metabolism in normal and diseased heart and a tool for noninvasively measuring metabolite reductions associated with the loss of viability.

AB - PURPOSE: To develop image-guided spatially localized magnetic resonance (MR) spectroscopy to provide a noninvasive quantitative probe of myocardial creatine kinase (CK) metabolism, and to use it to determine the extent of changes in CK energy metabolism in nonviable infarcted canine myocardium. MATERIALS AND METHODS: Water-referenced localized phosphorus and proton MR spectroscopy were combined in a single protocol to noninvasively measure phosphocreatine (PCr), adenosine triphosphate (ATP), and total of phosphorylated and unphosphorylated creatine (CR) concentrations and pH in the myocardium in six normal dogs and six dogs with surgically induced myocardial infarction. Unphosphorylated creatine and adenosine diphosphate (ADP) levels were calculated. The results were compared with biochemical measurements at postmortem biopsy. RESULTS: Significant reductions in PCr-to-ATP ratios (1.7 ± 0.3 [SD] vs 1 ± 0.4; P <.001), PCr (10.3 ± 2.1 vs 4.3 ± 2.0 μmol/g wet weight; P <.0001), ATP (6.4 ± 1.4 vs 3.7 ± 1.4 μmol/g wet weight; P <.001), and CR (24.7 ± 6.1 vs 6.3 ± 3.7; P <.0001) were measured noninvasively in infarcted, as compared with normal, tissue. Biopsy measurements confirmed infarct-related reductions observed at MR spectroscopy, although high-energy phosphate concentrations were lower at biopsy. ADP calculated from noninvasive MR spectroscopic measurements was 0.11 ± 0.07 μmol/g wet weight in normal myocardium. CONCLUSION: This combined phosphorus and proton MR spectroscopic approach provides a near-complete picture of in vivo myocardial CK metabolism in normal and diseased heart and a tool for noninvasively measuring metabolite reductions associated with the loss of viability.

KW - Animals Heart, MR, 511.121411, 511.121415

KW - Heart, MR spectroscopy, 511.12145

KW - Magnetic resonance (MR), Phosphorus studies, 511.121411, 511.121415, 511.12147

KW - Magnetic resonance (MR), spectroscopy, 511.12145

KW - Metabolism, 511.91

KW - Myocardium, infarction, 511.814

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M3 - Article

C2 - 11323465

AN - SCOPUS:0035055634

VL - 219

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EP - 418

JO - Radiology

JF - Radiology

SN - 0033-8419

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