Noninvasive assessment of cutaneous wound healing using ultrahigh-resolution optical coherence tomography

Michael J. Cobb; Yuchuan Chen; Robert A. Underwood; Marcia L. Usui; John Olerud; Xingde Li

doi:10.1117/1.2388152

Noninvasive assessment of cutaneous wound healing using ultrahigh-resolution optical coherence tomography

Michael J. Cobb, Yuchuan Chen, Robert A. Underwood, Marcia L. Usui, John Olerud, Xingde Li

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Ultrahigh-resolution optical coherence tomography (OCT) was used for noninvasive in vivo evaluation of the wound healing process. Cutaneous wounds were induced by 2.5-mm diameter fullthickness punch biopsies on the dorsal surface of seven mice. OCT imaging was performed to assess the structural characteristics associated with the healing process. The OCT results were compared to corresponding histology. Two automated quantitative analysis routines were implemented to identify the dermal-epidermal junction and segment the OCT images. Hallmarks of cutaneous wound healing such as wound size, epidermal migration, dermal-epidermal junction formation, and differences in wound composition were readily identified on the OCT images. Blister formation was also observed. Preliminary findings suggest OCT is a viable tool to noninvasively monitor wound healing in vivo.

Original language	English (US)
Article number	064002
Journal	Journal of biomedical optics
Volume	11
Issue number	6
DOIs	https://doi.org/10.1117/1.2388152
State	Published - Nov 2006
Externally published	Yes

Keywords

Image segmentation and imaging
Optical coherence tomography
Wound healing

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.2388152

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title = "Noninvasive assessment of cutaneous wound healing using ultrahigh-resolution optical coherence tomography",

abstract = "Ultrahigh-resolution optical coherence tomography (OCT) was used for noninvasive in vivo evaluation of the wound healing process. Cutaneous wounds were induced by 2.5-mm diameter fullthickness punch biopsies on the dorsal surface of seven mice. OCT imaging was performed to assess the structural characteristics associated with the healing process. The OCT results were compared to corresponding histology. Two automated quantitative analysis routines were implemented to identify the dermal-epidermal junction and segment the OCT images. Hallmarks of cutaneous wound healing such as wound size, epidermal migration, dermal-epidermal junction formation, and differences in wound composition were readily identified on the OCT images. Blister formation was also observed. Preliminary findings suggest OCT is a viable tool to noninvasively monitor wound healing in vivo.",

keywords = "Image segmentation and imaging, Optical coherence tomography, Wound healing",

author = "Cobb, {Michael J.} and Yuchuan Chen and Underwood, {Robert A.} and Usui, {Marcia L.} and John Olerud and Xingde Li",

note = "Funding Information: The authors would like to thank Dr. Yansong Gu for his assistance with the histology correlations. This research was supported in part by the Whitaker Foundation, National Institutes of Health, the National Institute of Dental and Craniofacial Research Predoctoral Training Fellowship (M. J. C.), and the National Science Foundation CAREER Award (X. D. L.).",

year = "2006",

month = nov,

doi = "10.1117/1.2388152",

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AU - Underwood, Robert A.

AU - Usui, Marcia L.

AU - Olerud, John

AU - Li, Xingde

N1 - Funding Information: The authors would like to thank Dr. Yansong Gu for his assistance with the histology correlations. This research was supported in part by the Whitaker Foundation, National Institutes of Health, the National Institute of Dental and Craniofacial Research Predoctoral Training Fellowship (M. J. C.), and the National Science Foundation CAREER Award (X. D. L.).

PY - 2006/11

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N2 - Ultrahigh-resolution optical coherence tomography (OCT) was used for noninvasive in vivo evaluation of the wound healing process. Cutaneous wounds were induced by 2.5-mm diameter fullthickness punch biopsies on the dorsal surface of seven mice. OCT imaging was performed to assess the structural characteristics associated with the healing process. The OCT results were compared to corresponding histology. Two automated quantitative analysis routines were implemented to identify the dermal-epidermal junction and segment the OCT images. Hallmarks of cutaneous wound healing such as wound size, epidermal migration, dermal-epidermal junction formation, and differences in wound composition were readily identified on the OCT images. Blister formation was also observed. Preliminary findings suggest OCT is a viable tool to noninvasively monitor wound healing in vivo.

AB - Ultrahigh-resolution optical coherence tomography (OCT) was used for noninvasive in vivo evaluation of the wound healing process. Cutaneous wounds were induced by 2.5-mm diameter fullthickness punch biopsies on the dorsal surface of seven mice. OCT imaging was performed to assess the structural characteristics associated with the healing process. The OCT results were compared to corresponding histology. Two automated quantitative analysis routines were implemented to identify the dermal-epidermal junction and segment the OCT images. Hallmarks of cutaneous wound healing such as wound size, epidermal migration, dermal-epidermal junction formation, and differences in wound composition were readily identified on the OCT images. Blister formation was also observed. Preliminary findings suggest OCT is a viable tool to noninvasively monitor wound healing in vivo.

KW - Image segmentation and imaging

KW - Optical coherence tomography

KW - Wound healing

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Noninvasive assessment of cutaneous wound healing using ultrahigh-resolution optical coherence tomography

Abstract

Keywords

ASJC Scopus subject areas

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