TY - JOUR
T1 - Noninflammatory comedones have greater diversity in microbiome and are more prone to biofilm formation than inflammatory lesions of acne vulgaris
AU - Loss, Manisha
AU - Thompson, Katherine G.
AU - Agostinho-Hunt, Alessandra
AU - James, Garth A.
AU - Mongodin, Emmanuel F.
AU - Rosenthal, Ian
AU - Cheng, Nancy
AU - Leung, Sherry
AU - Chien, Anna L.
AU - Kang, Sewon
N1 - Funding Information:
Funding for this study was provided by a grant from the American Acne and Rosacea Society (to Manisha Loss) and from the Provost Young Investigator Fund of the Johns Hopkins Department of Dermatology. The 16S rRNA gene sequencing performed in this study was supported by startup funds from the University of Maryland—School of Medicine to the Institute for Genome Sciences.
PY - 2020
Y1 - 2020
N2 - Background: The ability of Cutibacterium acnes strains to form biofilms has been correlated with their virulence. Objective: This study examined biofilm and skin microbiota in acne patients in order to understand their role in the development of acne lesions. Methods: Thin sections of punch biopsy specimens of (i) uninflamed comedones, (ii) inflammatory lesions, and (iii) uninvolved adjacent skin of acne patients were examined. Epiflourescence and confocal laser scanning microscopy were used for biofilm detection, and pyrosequencing with taxonomic classification of 16s rRNA gene amplicons was used for microbiota analysis. Results: Of the 39 skin specimens from patients with mild-moderate acne (n = 13) that were studied, nine (23%) contained biofilm. Among these specimens, biofilm was most frequently detected in comedones (55.6%) and less frequently in inflammatory papules (22.2%) and uninvolved skin (22.2%). Comedones demonstrated the highest mean alpha diversity of all the lesion subtypes. The relative abundance of Staphylococcus was significantly higher in comedones (11.400% ± 12.242%) compared to uninvolved skin (0.073% ± 0.185%, P = 0.024). Conclusions: The microenvironment of the comedone differs from that of inflammatory lesions and unaffected skin. The increased frequency of biofilm in comedones may account for the lack of host inflammatory response to these lesions.
AB - Background: The ability of Cutibacterium acnes strains to form biofilms has been correlated with their virulence. Objective: This study examined biofilm and skin microbiota in acne patients in order to understand their role in the development of acne lesions. Methods: Thin sections of punch biopsy specimens of (i) uninflamed comedones, (ii) inflammatory lesions, and (iii) uninvolved adjacent skin of acne patients were examined. Epiflourescence and confocal laser scanning microscopy were used for biofilm detection, and pyrosequencing with taxonomic classification of 16s rRNA gene amplicons was used for microbiota analysis. Results: Of the 39 skin specimens from patients with mild-moderate acne (n = 13) that were studied, nine (23%) contained biofilm. Among these specimens, biofilm was most frequently detected in comedones (55.6%) and less frequently in inflammatory papules (22.2%) and uninvolved skin (22.2%). Comedones demonstrated the highest mean alpha diversity of all the lesion subtypes. The relative abundance of Staphylococcus was significantly higher in comedones (11.400% ± 12.242%) compared to uninvolved skin (0.073% ± 0.185%, P = 0.024). Conclusions: The microenvironment of the comedone differs from that of inflammatory lesions and unaffected skin. The increased frequency of biofilm in comedones may account for the lack of host inflammatory response to these lesions.
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U2 - 10.1111/ijd.15308
DO - 10.1111/ijd.15308
M3 - Article
AN - SCOPUS:85097315272
JO - International Journal of Dermatology
JF - International Journal of Dermatology
SN - 0011-9059
ER -