Nonhuman sialic acid Neu5Gc is very low in human embryonic stem cell-derived neural precursors differentiated with B27/N2 and noggin: Implications for transplantation

Igor O. Nasonkin, Vassilis El Koliatsos

Research output: Contribution to journalArticle

Abstract

A concern recently has been raised that human embryonic stem cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1% in differentiating cells upon switching to B27/N2 medium.

Original languageEnglish (US)
Pages (from-to)525-529
Number of pages5
JournalExperimental Neurology
Volume201
Issue number2
DOIs
StatePublished - Oct 2006

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N-Acetylneuraminic Acid
Transplantation
Cell Line
Membranes
Human Embryonic Stem Cells

Keywords

  • Human embryonic stem cells
  • Immunogenicity
  • Neu5Gc
  • Neural differentiation
  • Sialic
  • Transplantation

ASJC Scopus subject areas

  • Neurology
  • Neuroscience(all)

Cite this

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abstract = "A concern recently has been raised that human embryonic stem cell (HESC) lines cultured with currently available methods may have limited clinical usefulness due to the immunogenicity of the nonhuman sialic acid Neu5Gc incorporated into their membranes during culturing. We find this concern has little relevance to neural differentiation protocols with B27/N2/noggin because of the gradual decline of Neu5Gc to less than 1{\%} in differentiating cells upon switching to B27/N2 medium.",
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