TY - JOUR
T1 - Nonclassical transpeptidases of Mycobacterium tuberculosis alter cell size, morphology, the cytosolic matrix, protein localization, virulence, and resistance to β-lactams
AU - Schoonmaker, Maia K.
AU - Bishai, William R.
AU - Lamichhanea, Gyanu
PY - 2014/4
Y1 - 2014/4
N2 - Virtually all bacteria possess a peptidoglycan layer that is essential for their growth and survival. The β-lactams, the most widely used class of antibiotics in human history, inhibit D, D-transpeptidases, which catalyze the final step in peptidoglycan biosynthesis. The existence of a second class of transpeptidases, the L, D-transpeptidases, was recently reported. Mycobacterium tuberculosis, an infectious pathogen that causes tuberculosis (TB), is known to possess as many as five proteins with L, D-transpeptidase activity. Here, for the first time, we demonstrate that loss of L, D-transpeptidases 1 and 2 of M. tuberculosis (LdtMt1 and LdtMt2) alters cell surface morphology, shape, size, organization of the intracellular matrix, sorting of some low-molecular-weight proteins that are targeted to the membrane or secreted, cellular physiology, growth, virulence, and resistance of M. tuberculosis to amoxicillin-clavulanate and vancomycin.
AB - Virtually all bacteria possess a peptidoglycan layer that is essential for their growth and survival. The β-lactams, the most widely used class of antibiotics in human history, inhibit D, D-transpeptidases, which catalyze the final step in peptidoglycan biosynthesis. The existence of a second class of transpeptidases, the L, D-transpeptidases, was recently reported. Mycobacterium tuberculosis, an infectious pathogen that causes tuberculosis (TB), is known to possess as many as five proteins with L, D-transpeptidase activity. Here, for the first time, we demonstrate that loss of L, D-transpeptidases 1 and 2 of M. tuberculosis (LdtMt1 and LdtMt2) alters cell surface morphology, shape, size, organization of the intracellular matrix, sorting of some low-molecular-weight proteins that are targeted to the membrane or secreted, cellular physiology, growth, virulence, and resistance of M. tuberculosis to amoxicillin-clavulanate and vancomycin.
UR - http://www.scopus.com/inward/record.url?scp=84895735604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84895735604&partnerID=8YFLogxK
U2 - 10.1128/JB.01396-13
DO - 10.1128/JB.01396-13
M3 - Article
C2 - 24464457
AN - SCOPUS:84895735604
SN - 0021-9193
VL - 196
SP - 1394
EP - 1402
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 7
ER -