Non-specific suppression of antigen-induced lymphocyte blastogenesis in Onchocerca volvulus infection in man

B. M. Greene, M. M. Fanning, J. J. Ellner

Research output: Contribution to journalArticle

Abstract

Lymphocyte blastogenic responses to O. volvulus antigen (Oncho Ag), SKSD, and the mitogen PHA were tested in three groups of persons: light to moderately infected persons (INF); previously exposed but uninfected persons (EXP) and normal controls (NC). The exposed group showed significant responsiveness to Oncho Ag (Δct/min = 6,002 ± 1,375), while the infected (Δct/min = 943 ± 418) and normal control (Δct/min = 428 ± 418) groups did not. The mean blastogenic response to SKSD were EXP, 8,644 ± 5,249; NC 6,039 ± 2,880; INF, 2,619 ± 1,012. The reduced reactivity in the INF group to Oncho Ag showed a significant correlation with reactivity to SKSD (P <0.05). To elucidate the mechanism of hyporesponsiveness in the infected group rigorous adherent cell depletion, by adherence to plastic followed by a nylon wool column, was utilized. When 20% plastic adherent cells were added back to the T cells prepared in this fashion, the mean blastogenic response to SKSD was significantly augmented (P <0.01). In contrast, the responsiveness to Oncho Ag was not significantly altered. The addition of indomethacin (1 μg/ml) or autologous plasma had no significant effect on reactivity to either SKSD or Oncho Ag. There were no significant differences in the mean reactivity of the three groups to PHA-M (Δct/min EXP 78,514 ± 12,564; INF 62,393 ± 14,447; NC 61,423 ± 4,465). These results suggest that O. volvulus infection is associated with decreased lymphocyte reactivity to both parasite related and unrelated antigens, and imply that the mechanism for the two types of hyporesponsiveness may be distinct. While a weakly adherent suppressor cell may account for non-specific hyporesponsiveness, the mechanism of parasite specific decreasd reactivity remains unknown.

Original languageEnglish (US)
Pages (from-to)259-263
Number of pages5
JournalClinical and Experimental Immunology
Volume52
Issue number2
StatePublished - 1983
Externally publishedYes

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Onchocerca volvulus
Lymphocyte Activation
Lymphocytes
Antigens
Infection
Plastics
Parasites
O Antigens
Wool
Nylons
Mitogens
Indomethacin
T-Lymphocytes
Light

ASJC Scopus subject areas

  • Immunology

Cite this

Non-specific suppression of antigen-induced lymphocyte blastogenesis in Onchocerca volvulus infection in man. / Greene, B. M.; Fanning, M. M.; Ellner, J. J.

In: Clinical and Experimental Immunology, Vol. 52, No. 2, 1983, p. 259-263.

Research output: Contribution to journalArticle

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abstract = "Lymphocyte blastogenic responses to O. volvulus antigen (Oncho Ag), SKSD, and the mitogen PHA were tested in three groups of persons: light to moderately infected persons (INF); previously exposed but uninfected persons (EXP) and normal controls (NC). The exposed group showed significant responsiveness to Oncho Ag (Δct/min = 6,002 ± 1,375), while the infected (Δct/min = 943 ± 418) and normal control (Δct/min = 428 ± 418) groups did not. The mean blastogenic response to SKSD were EXP, 8,644 ± 5,249; NC 6,039 ± 2,880; INF, 2,619 ± 1,012. The reduced reactivity in the INF group to Oncho Ag showed a significant correlation with reactivity to SKSD (P <0.05). To elucidate the mechanism of hyporesponsiveness in the infected group rigorous adherent cell depletion, by adherence to plastic followed by a nylon wool column, was utilized. When 20{\%} plastic adherent cells were added back to the T cells prepared in this fashion, the mean blastogenic response to SKSD was significantly augmented (P <0.01). In contrast, the responsiveness to Oncho Ag was not significantly altered. The addition of indomethacin (1 μg/ml) or autologous plasma had no significant effect on reactivity to either SKSD or Oncho Ag. There were no significant differences in the mean reactivity of the three groups to PHA-M (Δct/min EXP 78,514 ± 12,564; INF 62,393 ± 14,447; NC 61,423 ± 4,465). These results suggest that O. volvulus infection is associated with decreased lymphocyte reactivity to both parasite related and unrelated antigens, and imply that the mechanism for the two types of hyporesponsiveness may be distinct. While a weakly adherent suppressor cell may account for non-specific hyporesponsiveness, the mechanism of parasite specific decreasd reactivity remains unknown.",
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