Non-isotopic polymerase chain reaction methods for the detection of HIV-1 in Ugandan mothers and infants

J. Brooks Jackson, Christopher Ndugwa, Francis Mmiro, Peter Kataaha, Laura Guay, Elizabeth A. Dragon, Johanna Goldfarb, Karen Olness

Research output: Contribution to journalArticlepeer-review

Abstract

Two non-isotopic polymerase chain reaction (PCR) methods were evaluated by testing blood from 41 HIV-1 -seropositive and 16 HIV-1-seronegative Ugandan mothers and 56 of their children (aged 0.5-15.0 months). Amplification of HIV-1 sequences was performed in duplicate using a biotinylated primer pair to the gag region (SK 462-431) and nested primer pairs (JA 17-20) to the pol region of HIV-1. gag sequences were hybridized using a microtiter plate coated with the SK 102 probe followed by colorimetric detection using an avidin-horseradish peroxidase conjugate and tetramethylbenzidine/peroxide substrate, pol sequences were detected on agarose gel stained with ethidium bromide. Results of HIV-1 PCR analysis showed that 40 out of 41 (98%) seropositive mothers and 10 out of 29 (34%) seropositive children had detectable HIV-1 gag and pol sequences. None of the 16 seronegative mothers nor 27 seronegative or Western blot-indeterminate children had detectable HIV-1 sequences. Our results suggest that non-isotopic PCR methods are sensitive, specific, and potentially useful in the early diagnosis of HIV-1 infection in developed and developing countries.

Original languageEnglish (US)
Pages (from-to)1463-1467
Number of pages5
JournalAIDS
Volume5
Issue number12
StatePublished - Dec 1991
Externally publishedYes

Keywords

  • AIDS
  • HIV-1
  • HIV-1 vertical transmission
  • Non-isotopic
  • Polymerase chain reaction
  • Uganda

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

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