Non-invasive imaging of cardiac transgene expression with PET: Comparison of the human sodium/iodide symporter gene and HSV1-tk as the reporter gene

Masao Miyagawa, Martina Anton, Bettina Wagner, Roland Haubner, Michael Souvatzoglou, Bernd Gansbacher, Markus Schwaiger, Frank M. Bengel

Research output: Contribution to journalArticle

Abstract

Purpose: Genes encoding for intracellular enzymes or transmembrane proteins are suitable as reporters, but may differ in terms of applicability for cardiac imaging. The aim of this study was to compare the human sodium iodide symporter gene (hNIS) with the herpes simplex virus type 1 thymidine kinase gene (HSV1-tk) as the reporter gene in non-invasive imaging of cardiac transgene expression with positron emission tomography (PET). Methods: Equal doses of adenoviral vectors encoding for hNIS, wild-type HSV1-tk, mutant HSV1-sr39tk or LacZ as the control gene were directly injected into the myocardium of 34 animals. Two days later, dynamic PET was performed with a clinical scanner, using reporter probes specific for the respective reporter gene. Imaging with 13N-ammonia was also performed to identify cardiac regions of interest. Results: Kinetics differed significantly: 124I as the probe for hNIS showed rapid early uptake, remaining stable over time. Maximal myocardial concentration was 3.61±1.15%. The nucleoside 18F-FHBG, as the specific probe for HSV1-sr39tk, showed increasing uptake over time, but maximal accumulation was significantly lower (1.45±0.54%, P=0.0009). 124I-FIAU, as the specific probe for wild-type HSV1-tk, showed early uptake with subsequent washout. Maximal accumulation was lowest (0.63±0.23%, P

Original languageEnglish (US)
Pages (from-to)1108-1114
Number of pages7
JournalEuropean Journal of Nuclear Medicine and Molecular Imaging
Volume32
Issue number9
DOIs
StatePublished - Sep 2005
Externally publishedYes

Fingerprint

Thymidine Kinase
Human Herpesvirus 1
Transgenes
Reporter Genes
Positron-Emission Tomography
Genes
sodium-iodide symporter
Nucleosides
Ammonia
Myocardium
Enzymes

Keywords

  • Adenovirus
  • Gene therapy
  • HSV1-tk
  • Myocardium
  • Sodium iodide symporter

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Non-invasive imaging of cardiac transgene expression with PET : Comparison of the human sodium/iodide symporter gene and HSV1-tk as the reporter gene. / Miyagawa, Masao; Anton, Martina; Wagner, Bettina; Haubner, Roland; Souvatzoglou, Michael; Gansbacher, Bernd; Schwaiger, Markus; Bengel, Frank M.

In: European Journal of Nuclear Medicine and Molecular Imaging, Vol. 32, No. 9, 09.2005, p. 1108-1114.

Research output: Contribution to journalArticle

Miyagawa, Masao ; Anton, Martina ; Wagner, Bettina ; Haubner, Roland ; Souvatzoglou, Michael ; Gansbacher, Bernd ; Schwaiger, Markus ; Bengel, Frank M. / Non-invasive imaging of cardiac transgene expression with PET : Comparison of the human sodium/iodide symporter gene and HSV1-tk as the reporter gene. In: European Journal of Nuclear Medicine and Molecular Imaging. 2005 ; Vol. 32, No. 9. pp. 1108-1114.
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AU - Haubner, Roland

AU - Souvatzoglou, Michael

AU - Gansbacher, Bernd

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