Abstract
The highly conserved Wnt family of growth factors is essential for generating embryonic pattern in many animal species [1]. In the fruit fly Drosophila, most Writ-mediated patterning is performed by a single family member, Wingless (Wg), acting through its receptors Frizzled (Fz) and DFrizzled2 (Dfz2). In the ventral embryonic epidermis, Wg signaling generates two different cell-fate decisions: the production of diverse denticle types and the specification of naked cuticlle separating the denticle belts. Mutant alleles of wg disrupt these cellular decisions separately [2], suggesting that some aspect of ligand-receptor affinity influences cell-fate decisions, or that different receptor complexes mediate the distinct cellular responses. Here, we report that overexpression of Dfz2, but not Fz, rescues the mutant phenotype of wg(PE2), an allele that produces denticle diversity but no naked cuticle. Fz was able to substitute for Dfz2 only under conditions where the Wg ligand was present in excess. The wg(PE2) mutant phenotype was also sensitive to the dosage of glycosaminoglycans, suggesting that the mutant ligand is excluded from the receptor complex when proteoglycans are present. We conclude that wild-type Wg signaling requires efficient interaction between ligand and the Dfz2-proteoglycan receptor complex to promote the naked cuticle cell fate.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1127-1130 |
| Number of pages | 4 |
| Journal | Current Biology |
| Volume | 10 |
| Issue number | 18 |
| DOIs | |
| State | Published - Sep 21 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
Cite this
Non-equivalent roles of Drosophila Frizzled end Dfrizzled2 in embryonic Wingless signal transduction. / Moline, Melissa M.; Dierick, Herman A.; Pruette, Cozumel S; Bejsovec, Amy.
In: Current Biology, Vol. 10, No. 18, 21.09.2000, p. 1127-1130.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Non-equivalent roles of Drosophila Frizzled end Dfrizzled2 in embryonic Wingless signal transduction
AU - Moline, Melissa M.
AU - Dierick, Herman A.
AU - Pruette, Cozumel S
AU - Bejsovec, Amy
PY - 2000/9/21
Y1 - 2000/9/21
N2 - The highly conserved Wnt family of growth factors is essential for generating embryonic pattern in many animal species [1]. In the fruit fly Drosophila, most Writ-mediated patterning is performed by a single family member, Wingless (Wg), acting through its receptors Frizzled (Fz) and DFrizzled2 (Dfz2). In the ventral embryonic epidermis, Wg signaling generates two different cell-fate decisions: the production of diverse denticle types and the specification of naked cuticlle separating the denticle belts. Mutant alleles of wg disrupt these cellular decisions separately [2], suggesting that some aspect of ligand-receptor affinity influences cell-fate decisions, or that different receptor complexes mediate the distinct cellular responses. Here, we report that overexpression of Dfz2, but not Fz, rescues the mutant phenotype of wg(PE2), an allele that produces denticle diversity but no naked cuticle. Fz was able to substitute for Dfz2 only under conditions where the Wg ligand was present in excess. The wg(PE2) mutant phenotype was also sensitive to the dosage of glycosaminoglycans, suggesting that the mutant ligand is excluded from the receptor complex when proteoglycans are present. We conclude that wild-type Wg signaling requires efficient interaction between ligand and the Dfz2-proteoglycan receptor complex to promote the naked cuticle cell fate.
AB - The highly conserved Wnt family of growth factors is essential for generating embryonic pattern in many animal species [1]. In the fruit fly Drosophila, most Writ-mediated patterning is performed by a single family member, Wingless (Wg), acting through its receptors Frizzled (Fz) and DFrizzled2 (Dfz2). In the ventral embryonic epidermis, Wg signaling generates two different cell-fate decisions: the production of diverse denticle types and the specification of naked cuticlle separating the denticle belts. Mutant alleles of wg disrupt these cellular decisions separately [2], suggesting that some aspect of ligand-receptor affinity influences cell-fate decisions, or that different receptor complexes mediate the distinct cellular responses. Here, we report that overexpression of Dfz2, but not Fz, rescues the mutant phenotype of wg(PE2), an allele that produces denticle diversity but no naked cuticle. Fz was able to substitute for Dfz2 only under conditions where the Wg ligand was present in excess. The wg(PE2) mutant phenotype was also sensitive to the dosage of glycosaminoglycans, suggesting that the mutant ligand is excluded from the receptor complex when proteoglycans are present. We conclude that wild-type Wg signaling requires efficient interaction between ligand and the Dfz2-proteoglycan receptor complex to promote the naked cuticle cell fate.
UR - http://www.scopus.com/inward/record.url?scp=0034699321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034699321&partnerID=8YFLogxK
U2 - 10.1016/S0960-9822(00)00697-7
DO - 10.1016/S0960-9822(00)00697-7
M3 - Article
C2 - 10996794
AN - SCOPUS:0034699321
VL - 10
SP - 1127
EP - 1130
JO - Current Biology
JF - Current Biology
SN - 0960-9822
IS - 18
ER -