No role for human papillomavirus in esophageal squamous cell carcinoma in China

Jill Koshiol, Wen Qiang Wei, Aimee R. Kreimer, Wen Chen, Patti Gravitt, Jian Song Ren, Christian C. Abnet, Jian Bing Wang, Farin Kamangar, Dong Mei Lin, Magnus Von Knebel-Doeberitz, Yu Zhang, Raphael Viscidi, Guo Qing Wang, Maura L. Gillison, Mark J. Roth, Zhi Wei Dong, Esther Kim, Philip R. Taylor, You Lin QiaoSanford M. Dawsey

Research output: Contribution to journalArticle

Abstract

Certain regions of China have high rates of esophageal squamous cell carcinoma (ESCC). Previous studies of human papillomavirus (HPV), a proposed causal factor, have produced highly variable results. We attempted to evaluate HPV and ESCC more definitively using extreme care to prevent DNA contamination. We collected tissue and serum in China from 272 histopathologically-confirmed ESCC cases with rigorous attention to good molecular biology technique. We tested for HPV DNA in fresh-frozen tumor tissue using PCR with PGMY L1 consensus primers and HPV16 and 18 type-specific E6 and E7 primers, and in formalin-fixed paraffin-embedded tumor tissue using SPF10 L1 primers. In HPV-positive cases, we evaluated p16INK4a overexpression and HPV E6/E7 seropositivity as evidence of carcinogenic HPV activity. β-globin, and thus DNA, was adequate in 98.2% of the frozen tumor tissues (267/272). Of these, 99.6% (95% confidence interval (CI) 5 97.9-100.0%) were negative for HPV DNA by PGMY, and 100% (95% CI 5 98.6-100%) were negative by HPV16/18 E6/E7 PCR. In the corresponding formalin-fixed paraffin-embedded tumor specimens, 99.3% (95% CI 5 97.3-99.9%) were HPV negative by SPF10. By PGMY, 1 case tested weakly positive for HPV89, a noncancer causing HPV type. By SPF 10, 2 cases tested weakly positive: 1 for HPV16 and 1 for HPV31. No HPV DNA-positive case had evidence of HPV oncogene activity as measured by p16INK4a overexpression or E6/E7 seropositivity. This study provides the most definitive evidence to date that HPV is not involved in ESCC carcinogenesis in China. HPV DNA contamination cannot be ruled out as an explanation for high HPV prevalence in ESCC tissue studies with less stringent tissue procurement and processing protocols.

Original languageEnglish (US)
Pages (from-to)93-100
Number of pages8
JournalInternational Journal of Cancer
Volume127
Issue number1
DOIs
StatePublished - Jul 1 2010

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Keywords

  • Esophageal squamous cell carcinoma
  • Human papillomavirus

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Koshiol, J., Wei, W. Q., Kreimer, A. R., Chen, W., Gravitt, P., Ren, J. S., Abnet, C. C., Wang, J. B., Kamangar, F., Lin, D. M., Von Knebel-Doeberitz, M., Zhang, Y., Viscidi, R., Wang, G. Q., Gillison, M. L., Roth, M. J., Dong, Z. W., Kim, E., Taylor, P. R., ... Dawsey, S. M. (2010). No role for human papillomavirus in esophageal squamous cell carcinoma in China. International Journal of Cancer, 127(1), 93-100. https://doi.org/10.1002/ijc.25023