No allelic association of an exon 13 polymorphism of the Gsα gene to alcohol and/or drug dependence

Henry R. Kranzler, Mary E. McCaul, Joel Gelernter, Gary S. Wand

Research output: Contribution to journalArticle

Abstract

The adenylyl cyclase signal transduction system, a ubiquitous second messenger system, has been identified as a potential marker for genetic risk of alcohol and drug dependence. Using the polymerase chain reaction (PCR) to amplify exon 13 of the Gsα gene, two alleles were distinguished by denaturing gradient gel electrophoresis. One allele, designed A1, contained the previously published C in the codon for asparagine 371, while the second allele, designated A2, contains a C-T transition that conserves the asparagine residue at codon 371. The neutral polymorphism eliminates a Fok I restriction enzyme cleavage site, allowing use of restriction fragment length polymorphisms of PCR products to determine allelic frequency in 235 subjects with alcohol and/or drug dependence and in 85 control subjects. Since allele frequencies differ significantly by race, comparisons between affected individuals and controls were conducted separately for white and black groups. Within race, there were no significant differences in the frequency of the A2 allele among alcoholics, subjects dependent on cocaine or opioids, subjects dependent on these drugs and alcohol, and controls. We conclude that there is no association between alcohol and/or drug dependence and alleles of an exon 13 polymorphism of the Gsα gene in either black or white individuals.

Original languageEnglish (US)
Pages (from-to)309-316
Number of pages8
JournalAddiction Biology
Volume2
Issue number3
DOIs
StatePublished - Jan 1 1997

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology
  • Psychiatry and Mental health

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