NMR studies of glucose and alanine utilization and maternal-embryonic nutrient transfer in the smooth dogfish, Mustelus canis

Charles R. Graham, Calhoun Bond, V. P. Chacko, Julian Lombardi

Research output: Contribution to journalArticle

Abstract

Captive dogfish, Mustelus canis, were injected with saline containing 13C-enriched alanine or glucose and permitted to metabolize labeled substrates. Blood plasma and other body fluids were analyzed using high field 13C nuclear magnetic resonance (NMR) spectroscopy. Both injected glucose and alanine are converted to lactate with alanine conversion more rapid than that of glucose. 13C-enriched alanine and lactate appear in intracapsular fluids after injection of 13C-enriched alanine into the maternal blood vascular system. Studies involving the injection of 13C-enriched nutrients into the maternal circulation can yield information on metabolite utilization and embryonic nutrition in viviparous matrotrophs.

Original languageEnglish (US)
Pages (from-to)199-207
Number of pages9
JournalComparative Biochemistry and Physiology -- Part A: Physiology
Volume111
Issue number2
DOIs
StatePublished - Jun 1995

Keywords

  • Alanine
  • Dogfish
  • Gestation
  • Glucose
  • Metabolism
  • Mustelus
  • NMR
  • Shark
  • Spectroscopy
  • Viviparity

ASJC Scopus subject areas

  • Physiology

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