NMDA Receptor Activation Underlies the Loss of Spinal Dorsal Horn Neurons and the Transition to Persistent Pain after Peripheral Nerve Injury

Perrine Inquimbert, Martin Moll, Alban Latremoliere, Chi Kun Tong, John Whang, Gregory F. Sheehan, Brendan M. Smith, Erica Korb, Maria C.P. Athié, Olusegun Babaniyi, Nader Ghasemlou, Yuchio Yanagawa, C. David Allis, Patrick R. Hof, Joachim Scholz

Research output: Contribution to journalArticle

Abstract

Peripheral nerve lesions provoke apoptosis in the dorsal horn of the spinal cord. The cause of cell death, the involvement of neurons, and the relevance for the processing of somatosensory information are controversial. Here, we demonstrate in a mouse model of sciatic nerve injury that glutamate-induced neurodegeneration and loss of γ-aminobutyric acid (GABA)ergic interneurons in the superficial dorsal horn promote the transition from acute to chronic neuropathic pain. Conditional deletion of Grin1, the essential subunit of N-methyl-D-aspartate-type glutamate receptors (NMDARs), protects dorsal horn neurons from excitotoxicity and preserves GABAergic inhibition. Mice deficient in functional NMDARs exhibit normal nociceptive responses and acute pain after nerve injury, but this initial increase in pain sensitivity is reversible. Eliminating NMDARs fully prevents persistent pain-like behavior. Reduced pain in mice lacking proapoptotic Bax confirmed the significance of neurodegeneration. We conclude that NMDAR-mediated neuron death contributes to the development of chronic neuropathic pain. Dorsal horn neurons process somatosensory information, including pain. Inquimbert et al. utilized spatially restricted Grin1 knockout to show that NMDA-receptor-mediated excitatory input causes the degeneration of some dorsal horn neurons after nerve injury. Irreversible loss of GABAergic interneurons leads to a deficit in inhibition that promotes persistent pain hypersensitivity.

Original languageEnglish (US)
Pages (from-to)2678-2689
Number of pages12
JournalCell Reports
Volume23
Issue number9
DOIs
StatePublished - May 29 2018

Fingerprint

Posterior Horn Cells
Peripheral Nerve Injuries
N-Methyl-D-Aspartate Receptors
Neurons
Glutamate Receptors
N-Methylaspartate
Chemical activation
Pain
Neuralgia
Interneurons
Chronic Pain
Wounds and Injuries
Aminobutyrates
Nociceptive Pain
Acute Pain
Sciatic Nerve
Cell death
Automatic Data Processing
Peripheral Nerves
gamma-Aminobutyric Acid

Keywords

  • chronic pain
  • disinhibition
  • dorsal horn
  • excitotoxicity
  • nerve injury
  • neuropathic pain
  • NMDA receptor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

NMDA Receptor Activation Underlies the Loss of Spinal Dorsal Horn Neurons and the Transition to Persistent Pain after Peripheral Nerve Injury. / Inquimbert, Perrine; Moll, Martin; Latremoliere, Alban; Tong, Chi Kun; Whang, John; Sheehan, Gregory F.; Smith, Brendan M.; Korb, Erica; Athié, Maria C.P.; Babaniyi, Olusegun; Ghasemlou, Nader; Yanagawa, Yuchio; Allis, C. David; Hof, Patrick R.; Scholz, Joachim.

In: Cell Reports, Vol. 23, No. 9, 29.05.2018, p. 2678-2689.

Research output: Contribution to journalArticle

Inquimbert, P, Moll, M, Latremoliere, A, Tong, CK, Whang, J, Sheehan, GF, Smith, BM, Korb, E, Athié, MCP, Babaniyi, O, Ghasemlou, N, Yanagawa, Y, Allis, CD, Hof, PR & Scholz, J 2018, 'NMDA Receptor Activation Underlies the Loss of Spinal Dorsal Horn Neurons and the Transition to Persistent Pain after Peripheral Nerve Injury', Cell Reports, vol. 23, no. 9, pp. 2678-2689. https://doi.org/10.1016/j.celrep.2018.04.107
Inquimbert, Perrine ; Moll, Martin ; Latremoliere, Alban ; Tong, Chi Kun ; Whang, John ; Sheehan, Gregory F. ; Smith, Brendan M. ; Korb, Erica ; Athié, Maria C.P. ; Babaniyi, Olusegun ; Ghasemlou, Nader ; Yanagawa, Yuchio ; Allis, C. David ; Hof, Patrick R. ; Scholz, Joachim. / NMDA Receptor Activation Underlies the Loss of Spinal Dorsal Horn Neurons and the Transition to Persistent Pain after Peripheral Nerve Injury. In: Cell Reports. 2018 ; Vol. 23, No. 9. pp. 2678-2689.
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