NMDA inhibitors cause apoptosis of pyramidal neurons in mature piriform cortex: Evidence for a nitric oxide-mediated effect involving inhibitory interneurons

Lijun Zhou, Annie M. Welsh, David Chen, Vassilis El Koliatsos

Research output: Contribution to journalArticle

Abstract

Pyramidal relay neurons in limbic cortex are vulnerable to denervation lesions, i.e. pyramidal neurons in layer IIα of piriform cortex undergo transsynaptic apoptosis after lesions that interrupt their inputs from the olfactory bulb. We have previously established the role of inhibitory interneurons in elaborating signals that lead to the apoptosis of projection neurons in these lesion models, i.e. the upregulation of neuronal NOS and release of nitric oxide. Thus, we have proposed that cortical interneurons play an essential role in transducing injury to degenerative effects for nearby pyramidal neurons. In the present study, we extend the previous findings to a toxic model of degeneration of pyramidal neurons in the adult paralimbic cortex, i.e. after exposure to the NMDA channel blocker MK801. Our findings indicate that treatment of adult rats with MK801 in doses previously found to cause alterations in pyramidal neurons of the retrosplenial cortex (5 mg/kg) results in an active caspase 3 (+), ultrastructurally apoptotic type of cell death involving the same projection neurons of layer IIα that are also vulnerable to bulbotomy lesions. Interneurons of layer I are induced by MK801 treatment to higher levels of nNOS expression and the selective nNOS inhibitor BRNI ameliorates pyramidal cell apoptosis caused by MK801. Our results indicate that certain pyramidal neurons in piriform cortex are very sensitive to NMDA blockade as they are to disconnection from modality-specific afferents and that inhibitory interneurons play significant roles in mediating various types of pro-apoptotic insults to cortical projection neurons via nNOS/NO signaling.

Original languageEnglish (US)
Pages (from-to)1528-1537
Number of pages10
JournalNeuropharmacology
Volume52
Issue number7
DOIs
StatePublished - Jun 2007

Fingerprint

Pyramidal Cells
Interneurons
N-Methylaspartate
Nitric Oxide
Apoptosis
Neurons
Poisons
Olfactory Bulb
Denervation
Piriform Cortex
Caspase 3
Cell Death
Up-Regulation
Wounds and Injuries

Keywords

  • Excitotoxicity
  • GABA
  • Glutamate
  • Limbic system
  • Neuronal cell death
  • Olfactory

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

Cite this

NMDA inhibitors cause apoptosis of pyramidal neurons in mature piriform cortex : Evidence for a nitric oxide-mediated effect involving inhibitory interneurons. / Zhou, Lijun; Welsh, Annie M.; Chen, David; Koliatsos, Vassilis El.

In: Neuropharmacology, Vol. 52, No. 7, 06.2007, p. 1528-1537.

Research output: Contribution to journalArticle

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