Nm23-transfected MDA-MB-435 human breast carcinoma cells form tumors with altered phospholipid metabolism and pH: A 31P nuclear magnetic resonance study in vivo and vitro

Zaver M. Bhujwalla, Eric O. Aboagye, Robert J. Gillies, V. P. Chacko, Charmaine E. Mendola, Joseph M. Backer

Research output: Contribution to journalArticle

Abstract

Nm23 genes are involved in the control of the metastatic potential of breast carcinoma cells. To understand the impact of nm23 genes on tumor physiology and metabolism, a 31P nuclear magnetic resonance (NMR) spectroscopic study was performed on tumors formed in the mammary fat pad of severe combined immunodeficiency mice by MDA-MB-3435 human breast carcinoma cells transfected with cDNA encoding wild type nm23-H1 and nm23-H2 proteins. Tumors formed by MDA-MB-435 cells transfected with vector alone were used as controls. All transgene tumors exhibited significantly higher levels of phosphodiester (PDE) compounds relative to phosphomonoester (PME) compounds in vivo compared with control tumors. Similar differences in PDE and PME also were observed for spectra obtained from cells growing in culture. Intracellular pH was significantly lower and extracellular pH was significantly higher for transgene tumors compared with control tumors. Histologic analysis of lung sections confirmed reductions in incidence, number, and size of metastatic nodules for animals bearing transgene tumors. These results suggest that nm23 genes may affect suppression of metastasis through phospholipid-mediated signaling and cellular pH regulation.

Original languageEnglish (US)
Pages (from-to)897-903
Number of pages7
JournalMagnetic resonance in medicine
Volume41
Issue number5
DOIs
StatePublished - May 17 1999

Keywords

  • Human breast carcinoma metastasis
  • Nonmetastatic 23 transfection
  • P nuclear magnetic resonance spectroscopy
  • Phospholipid metabolism and pH

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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