NK cell-induced cytotoxicity is dependent on a Ca2+ increase in the target

David McConkey, Sek C. Chow, Sten Orrenius, Mikael Jondal

Research output: Contribution to journalArticle

Abstract

In previous work we showed that programed cell death (PCD) in thymocytes is mediated by a sustained increase in cytosolic Ca2+ concentration, resulting in the activation of an endogenous endonuclease, DNA fragmentation, and cell death. In this study we investigated the roles of Ca2+ and DNA fragmentation in target cell killing by natural killer (NK) cells. The effector cells induced a rapid, sustained increase in cytosolic Ca2+ concentration in Jurkat target cells. Buffering the target cell cytosolic Ca2+ with the Ca2+-selective dye, quin-2, prevented target cell killing. Extensive DNA fragmentation was associated with killing in every target tested, and this response was also blocked by quin-2. The endonuclease inhibitor, aurintricarboxylic acid, inhibited both DNA fragmentation and killing without influencing the Ca2+ increase in target cells. Thus, it is concluded that NK cell killing depends on a Ca2+ increase and appears to involve endogenous endonuclease activation in target cells.

Original languageEnglish (US)
Pages (from-to)2661-2664
Number of pages4
JournalFASEB Journal
Volume4
Issue number9
StatePublished - Jun 1990
Externally publishedYes

Fingerprint

natural killer cells
Cytotoxicity
Natural Killer Cells
cytotoxicity
Endonucleases
Cells
DNA Fragmentation
Cell death
calcium
DNA fragmentation
DNA
Aurintricarboxylic Acid
Chemical activation
Deoxyribonuclease I
cells
Cell Death
Coloring Agents
cell death
Jurkat Cells
Thymocytes

Keywords

  • Aurintricarboxylic acid
  • Calcium buffering
  • Endonuclease activation
  • Programed cell death

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

McConkey, D., Chow, S. C., Orrenius, S., & Jondal, M. (1990). NK cell-induced cytotoxicity is dependent on a Ca2+ increase in the target. FASEB Journal, 4(9), 2661-2664.

NK cell-induced cytotoxicity is dependent on a Ca2+ increase in the target. / McConkey, David; Chow, Sek C.; Orrenius, Sten; Jondal, Mikael.

In: FASEB Journal, Vol. 4, No. 9, 06.1990, p. 2661-2664.

Research output: Contribution to journalArticle

McConkey, D, Chow, SC, Orrenius, S & Jondal, M 1990, 'NK cell-induced cytotoxicity is dependent on a Ca2+ increase in the target', FASEB Journal, vol. 4, no. 9, pp. 2661-2664.
McConkey, David ; Chow, Sek C. ; Orrenius, Sten ; Jondal, Mikael. / NK cell-induced cytotoxicity is dependent on a Ca2+ increase in the target. In: FASEB Journal. 1990 ; Vol. 4, No. 9. pp. 2661-2664.
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