NK activity during graft-versus-host disease and graft rejection in rats following intestinal semiallogenic and allogenic transplantation with or without mesenteric lymphadenectomy

Ermenegildo E. Frezza, Giorgio E. Gerunda, Ambrogio Fassina, Roberto DeFranchis, Roberto Biffi, Ahmet Gurakar, Stefano Fagiuoli, Alvise Maffei Faccioli, David H. Van Thiel

Research output: Contribution to journalArticle

Abstract

Graft-versus-host disease (GVHD) and graft rejection are major problems following intestinal transplantation (IT). Natural killer (NK) cells may be important effector cells in both conditions. In this study, Sprague-Dawley (SD) or SD-Brown Norway (BN) F1 rat intestine was transplanted into BN recipients with and without associated graft mesenteric lymphadenectomy (GML). Cyclosporine (15 mg/kg day) was administered to all animals. Pieces of the intestinal graft were examined 4 days posttransplant and again at death. NK activity calculated using intestinal intraepithelial lymphocytes (IL) was determined utilizing an 18-hr cytotoxic assay assessing 51Cr release and the results are reported as lytic units. YAC-1 cells were used as the target. NK activity was reduced 4 days after IT both in native (8.02±0.64) and in grafted bowel (3.14±1.51), with histological evidence of rejection as compared with that of control bowel in ungrafted rats (21.1 ±2.14). Survival was increased, on mean, a total of 6 days with the addition of GML in both semiallogenic and allogenic transplanted rats. At the time of death, the NK activity in the native bowel had increased (17.1 ±3.02) and histologic evidence of GVHD was present. These data suggest that: (1) NK cells are important in GVHD and (2) both semiallogenic and allogenic transplants survive longer if they are combined with GML (P≤0.05 and P≤0.01, respectively).

Original languageEnglish (US)
Pages (from-to)698-701
Number of pages4
JournalTransplantation
Volume58
Issue number6
StatePublished - Sep 27 1994
Externally publishedYes

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Homologous Transplantation
Graft Rejection
Graft vs Host Disease
Lymph Node Excision
Transplants
Natural Killer Cells
Transplantation
Norway
Cyclosporine
Intestines
Lymphocytes

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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NK activity during graft-versus-host disease and graft rejection in rats following intestinal semiallogenic and allogenic transplantation with or without mesenteric lymphadenectomy. / Frezza, Ermenegildo E.; Gerunda, Giorgio E.; Fassina, Ambrogio; DeFranchis, Roberto; Biffi, Roberto; Gurakar, Ahmet; Fagiuoli, Stefano; Faccioli, Alvise Maffei; Van Thiel, David H.

In: Transplantation, Vol. 58, No. 6, 27.09.1994, p. 698-701.

Research output: Contribution to journalArticle

Frezza, EE, Gerunda, GE, Fassina, A, DeFranchis, R, Biffi, R, Gurakar, A, Fagiuoli, S, Faccioli, AM & Van Thiel, DH 1994, 'NK activity during graft-versus-host disease and graft rejection in rats following intestinal semiallogenic and allogenic transplantation with or without mesenteric lymphadenectomy', Transplantation, vol. 58, no. 6, pp. 698-701.
Frezza, Ermenegildo E. ; Gerunda, Giorgio E. ; Fassina, Ambrogio ; DeFranchis, Roberto ; Biffi, Roberto ; Gurakar, Ahmet ; Fagiuoli, Stefano ; Faccioli, Alvise Maffei ; Van Thiel, David H. / NK activity during graft-versus-host disease and graft rejection in rats following intestinal semiallogenic and allogenic transplantation with or without mesenteric lymphadenectomy. In: Transplantation. 1994 ; Vol. 58, No. 6. pp. 698-701.
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abstract = "Graft-versus-host disease (GVHD) and graft rejection are major problems following intestinal transplantation (IT). Natural killer (NK) cells may be important effector cells in both conditions. In this study, Sprague-Dawley (SD) or SD-Brown Norway (BN) F1 rat intestine was transplanted into BN recipients with and without associated graft mesenteric lymphadenectomy (GML). Cyclosporine (15 mg/kg day) was administered to all animals. Pieces of the intestinal graft were examined 4 days posttransplant and again at death. NK activity calculated using intestinal intraepithelial lymphocytes (IL) was determined utilizing an 18-hr cytotoxic assay assessing 51Cr release and the results are reported as lytic units. YAC-1 cells were used as the target. NK activity was reduced 4 days after IT both in native (8.02±0.64) and in grafted bowel (3.14±1.51), with histological evidence of rejection as compared with that of control bowel in ungrafted rats (21.1 ±2.14). Survival was increased, on mean, a total of 6 days with the addition of GML in both semiallogenic and allogenic transplanted rats. At the time of death, the NK activity in the native bowel had increased (17.1 ±3.02) and histologic evidence of GVHD was present. These data suggest that: (1) NK cells are important in GVHD and (2) both semiallogenic and allogenic transplants survive longer if they are combined with GML (P≤0.05 and P≤0.01, respectively).",
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AU - Frezza, Ermenegildo E.

AU - Gerunda, Giorgio E.

AU - Fassina, Ambrogio

AU - DeFranchis, Roberto

AU - Biffi, Roberto

AU - Gurakar, Ahmet

AU - Fagiuoli, Stefano

AU - Faccioli, Alvise Maffei

AU - Van Thiel, David H.

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Y1 - 1994/9/27

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