Nitroxyl enhances myocyte Ca²⁺ transients by exclusively targeting SR Ca²⁺-cycling

Nitroxyl (HNO), the 1-electron reduction product of nitric oxide, improves myocardial contraction in normal and failing hearts. Here we test whether the HNO donor Angeli's salt (AS) will change myocyte action potential (AP) waveform by altering the L-type Ca²⁺ current (ICa) and contrast the contractile effects of HNO with that of the hydroxyl radical (.OH) and nitrite (NO2-), two potential breakdown products of AS. We confirmed the positive effect of AS/HNO on basal cardiomyocyte function, as opposed to the detrimental effect of.OH and the negligible effect of NO2-. Upon examination of the myocyte AP, we observed no change in resting membrane potential or AP duration to 20% repolarization with AS/HNO, whereas AP duration to 90% repolarization was slightly prolonged. However, perfusion with AS/HNO did not elicit a change in basal ICa, but did hasten ICa inactivation. Upon further examination of the SR, the AS/HNO-induced increase in cardiomyocyte Ca²⁺ transients was abolished with inhibition of SR Ca²⁺-cycling. Therefore, the HNO-induced increase in Ca²⁺ transients results exclusively from changes in SR Ca²⁺-cycling, and not from ICa.