Nitroreductase-mediated cell ablation in transgenic zebrafish embryos.

Harshan Pisharath, Michael J. Parsons

Research output: Contribution to journalArticlepeer-review

Abstract

Prodrug dependent cell ablation is a method that allows inducible and spatially restricted cell destruction. We describe transgenic methods to express the Escherichia coli nfsB in a tissue restricted manner in the zebrafish. This bacterial gene encodes a nitroreductase (NTR) enzyme that can render prodrugs such as metronidazole (Met) cytotoxic. Using the expression of NTR fused to a fluorescent protein, one can simultaneously make cells susceptible to prodrug treatment and visualize cell ablation as it occurs.

Original languageEnglish (US)
Pages (from-to)133-143
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume546
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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