Abstract
Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C
Original language | English (US) |
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Pages (from-to) | 271-282 |
Number of pages | 12 |
Journal | Journal of Biomolecular NMR |
Volume | 47 |
Issue number | 4 |
DOIs | |
State | Published - Aug 2010 |
Keywords
- CAN
- CON
- HSQC
- N direct detection
- Nuclear magnetic resonance (NMR)
- Resonance assignment
ASJC Scopus subject areas
- Biochemistry
- Spectroscopy