Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments

Koh Takeuchi; Gregory Heffron; Zhen Yu J. Sun; Dominique P. Frueh; Gerhard Wagner

doi:10.1007/s10858-010-9430-z

Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments

Koh Takeuchi, Gregory Heffron, Zhen Yu J. Sun, Dominique P. Frueh, Gerhard Wagner

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as ¹³C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel ¹⁵N direct-detection experiments. The CAN experiment sequentially connects amide ¹⁵N resonances using ¹³Cα chemical shift matching, and the CON experiment connects the preceding ¹³C

Original language	English (US)
Pages (from-to)	271-282
Number of pages	12
Journal	Journal of Biomolecular NMR
Volume	47
Issue number	4
DOIs	https://doi.org/10.1007/s10858-010-9430-z
State	Published - Aug 2010

Keywords

CAN
CON
HSQC
N direct detection
Nuclear magnetic resonance (NMR)
Resonance assignment

ASJC Scopus subject areas

Biochemistry
Spectroscopy

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10.1007/s10858-010-9430-z

Cite this

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title = "Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments",

abstract = "Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C",

keywords = "CAN, CON, HSQC, N direct detection, Nuclear magnetic resonance (NMR), Resonance assignment",

author = "Koh Takeuchi and Gregory Heffron and Sun, {Zhen Yu J.} and Frueh, {Dominique P.} and Gerhard Wagner",

note = "Funding Information: Acknowledgments This work was supported by the NIH (grants AI37581, GM47467 and EB 002026).",

year = "2010",

month = aug,

doi = "10.1007/s10858-010-9430-z",

language = "English (US)",

volume = "47",

pages = "271--282",

journal = "Journal of Biomolecular NMR",

issn = "0925-2738",

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T1 - Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments

AU - Takeuchi, Koh

AU - Heffron, Gregory

AU - Sun, Zhen Yu J.

AU - Frueh, Dominique P.

AU - Wagner, Gerhard

N1 - Funding Information: Acknowledgments This work was supported by the NIH (grants AI37581, GM47467 and EB 002026).

PY - 2010/8

Y1 - 2010/8

N2 - Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C

AB - Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C

KW - CAN

KW - CON

KW - HSQC

KW - N direct detection

KW - Nuclear magnetic resonance (NMR)

KW - Resonance assignment

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EP - 282

JO - Journal of Biomolecular NMR

JF - Journal of Biomolecular NMR

SN - 0925-2738

IS - 4

ER -

Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments

Abstract

Keywords

ASJC Scopus subject areas

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