Nitrogen-detected CAN and CON experiments as alternative experiments for main chain NMR resonance assignments

Koh Takeuchi, Gregory Heffron, Zhen Yu J. Sun, Dominique P. Frueh, Gerhard Wagner

Research output: Contribution to journalArticlepeer-review

Abstract

Heteronuclear direct-detection experiments, which utilize the slower relaxation properties of low γ nuclei, such as 13C have recently been proposed for sequence-specific assignment and structural analyses of large, unstructured, and/or paramagnetic proteins. Here we present two novel 15N direct-detection experiments. The CAN experiment sequentially connects amide 15N resonances using 13Cα chemical shift matching, and the CON experiment connects the preceding 13C

Original languageEnglish (US)
Pages (from-to)271-282
Number of pages12
JournalJournal of Biomolecular NMR
Volume47
Issue number4
DOIs
StatePublished - Aug 2010

Keywords

  • CAN
  • CON
  • HSQC
  • N direct detection
  • Nuclear magnetic resonance (NMR)
  • Resonance assignment

ASJC Scopus subject areas

  • Biochemistry
  • Spectroscopy

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