Nitric oxide synthase inhibition reduces caudate injury following transient focal ischemia in cats

Toshiaki Nishikawa, Jeffrey R. Kirsch, Raymond C Koehler, Masayuki Miyabe, Richard J. Traystman

Research output: Contribution to journalArticle

Abstract

Background and Purpose We tested the hypothesis that inhibiting nitric oxide production either before or during transient focal ischemia affects early postischemic brain injury. Methods Halothane-anesthetized cats underwent 1 hour of left middle cerebral artery occlusion plus 3 hours of reperfusion. Pretreatment groups received either intravenous Nω-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg, n=10) or an equal volume of diluent (10 mL saline, n=10) over 30 minutes before ischemia. Posttreatment groups received intravenous L-NAME (10 mg/kg) over 30 minutes from 45 minutes of ischemia to 15 minutes of reperfusion (n=10) or intravenous L-NAME (10 mg/kg) plus L-arginine (200 mg/kg) over the same period followed by continuous L-arginine infusion (200 mg/kg per hour) for the remainder of reperfusion (n=10). Results Microsphere-determined blood flow to ipsilateral caudate nucleus and inferior temporal cortex decreased to the same extent during ischemia and recovered to the same extent during reperfusion in the four groups. Triphenyltetrazolium-determined injury volume of ipsilateral caudate nucleus in cats treated with L-NAME before or during ischemia (42±7% and 42±3% of caudate nucleus, respectively; mean±SE) was less (P

Original languageEnglish (US)
Pages (from-to)877-885
Number of pages9
JournalStroke
Volume25
Issue number4
StatePublished - 1994

Fingerprint

Nitric Oxide Synthase
NG-Nitroarginine Methyl Ester
Cats
Ischemia
Reperfusion
Caudate Nucleus
Wounds and Injuries
Arginine
Middle Cerebral Artery Infarction
Halothane
Temporal Lobe
Microspheres
Brain Injuries
Nitric Oxide

Keywords

  • Cats
  • Cerebral blood flow
  • Evoked potentials, somatosensory
  • Nitric oxide

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Clinical Neurology
  • Advanced and Specialized Nursing
  • Neuroscience(all)

Cite this

Nishikawa, T., Kirsch, J. R., Koehler, R. C., Miyabe, M., & Traystman, R. J. (1994). Nitric oxide synthase inhibition reduces caudate injury following transient focal ischemia in cats. Stroke, 25(4), 877-885.

Nitric oxide synthase inhibition reduces caudate injury following transient focal ischemia in cats. / Nishikawa, Toshiaki; Kirsch, Jeffrey R.; Koehler, Raymond C; Miyabe, Masayuki; Traystman, Richard J.

In: Stroke, Vol. 25, No. 4, 1994, p. 877-885.

Research output: Contribution to journalArticle

Nishikawa, T, Kirsch, JR, Koehler, RC, Miyabe, M & Traystman, RJ 1994, 'Nitric oxide synthase inhibition reduces caudate injury following transient focal ischemia in cats', Stroke, vol. 25, no. 4, pp. 877-885.
Nishikawa, Toshiaki ; Kirsch, Jeffrey R. ; Koehler, Raymond C ; Miyabe, Masayuki ; Traystman, Richard J. / Nitric oxide synthase inhibition reduces caudate injury following transient focal ischemia in cats. In: Stroke. 1994 ; Vol. 25, No. 4. pp. 877-885.
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