Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor

Kenji Matsushita, Craig N. Morrell, Beatrice Cambien, Shui Xiang Yang, Munekazu Yamakuchi, Clare Bao, Makoto R. Hara, Richard A. Quick, Wangsen Cao, Brian O'Rourke, John M. Lowenstein, Jonathan Pevsner, Denisa D. Wagner, Charles J. Lowenstein

Research output: Contribution to journalArticlepeer-review

Abstract

Nitric oxide (NO) inhibits vascular inflammation, but the molecular basis for its anti-inflammatory properties is unknown. We show that NO inhibits exocytosis of Weibel-Palade bodies, endothelial granules that mediate vascular inflammation and thrombosis, by regulating the activity of N-ethylmaleimide- sensitive factor (NSF). NO inhibits NSF disassembly of soluble NSF attachment protein receptor (SNARE) complexes by nitrosylating critical cysteine residues of NSF. NO may regulate exocytosis in a variety of physiological processes, including vascular inflammation, neurotransmission, thrombosis, and cytotoxic T lymphocyte cell killing.

Original languageEnglish (US)
Pages (from-to)139-150
Number of pages12
JournalCell
Volume115
Issue number2
DOIs
StatePublished - Oct 17 2003

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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