Nitric oxide redox reactions and red cell biology

Joseph M. Rifkind, Nagababu Enika, Somasundaram Ramasamy

Research output: Contribution to journalArticle

Abstract

Three hypotheses explain a role for red blood cells (RBCs) in delivering NO to the vasculature: (a) "the SNOHb hypothesis" involves the uptake of NO by RBCs with NO transferred from the heme to the β-93 thiol in the R quaternary conformation, followed by the release to membrane thiols in the T quaternary conformation; and (b and c) "the nitrite hypotheses" bypass the intrinsic difficulties of transporting the highly reactive NO, by reutilizing the nitrite formed when NO reacts with oxygen. Deoxyhemoglobin reduces this nitrite back to NO. The distinction between the two nitrite mechanisms depends on the importance of intermediate species formed during nitrite reduction. Without bioactive intermediates, the NO must be immediately released to avoid binding to deoxyhemoglobin. The "nitrite intermediate hypothesis" enables the RBCs to store a pool of potentially bioactive NO until it is released from the cell. In this review, we critically compare these different proposals for the transport/delivery of NO by RBCs. We also compare the redox properties in the RBCs associated with NO with the redox properties associated with oxygen.

Original languageEnglish (US)
Pages (from-to)1193-1203
Number of pages11
JournalAntioxidants and Redox Signaling
Volume8
Issue number7-8
DOIs
StatePublished - Jul 2006
Externally publishedYes

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Cytology
Redox reactions
Nitrites
Oxidation-Reduction
Cell Biology
Nitric Oxide
Blood
Erythrocytes
Sulfhydryl Compounds
Conformations
Oxygen
Heme
Cells
Membranes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nitric oxide redox reactions and red cell biology. / Rifkind, Joseph M.; Enika, Nagababu; Ramasamy, Somasundaram.

In: Antioxidants and Redox Signaling, Vol. 8, No. 7-8, 07.2006, p. 1193-1203.

Research output: Contribution to journalArticle

Rifkind, JM, Enika, N & Ramasamy, S 2006, 'Nitric oxide redox reactions and red cell biology', Antioxidants and Redox Signaling, vol. 8, no. 7-8, pp. 1193-1203. https://doi.org/10.1089/ars.2006.8.1193
Rifkind, Joseph M. ; Enika, Nagababu ; Ramasamy, Somasundaram. / Nitric oxide redox reactions and red cell biology. In: Antioxidants and Redox Signaling. 2006 ; Vol. 8, No. 7-8. pp. 1193-1203.
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