Nitric oxide is a mediator of the late-phase response in an animal model of nasal allergy

William F. Durland, Andrew P Lane, Kimberly W. Durland, Timothy L. Smith, Kenneth L. Johnson, Jiri Prazma, Harold C. Pillsbury

Research output: Contribution to journalArticle

Abstract

The presence of nitric oxide (NO) in the nose is well documented; however, the role of this molecule in nasal physiology is still poorly understood. Our laboratory has previously demonstrated that NO is a mediator of the immediate secretory response to an intranasal histamine challenge in a rat model of nasal allergy. Histamine challenge, however, does not elicit a late-phase response (LPR). To study the role of NO in the LPR, we developed a model of nasal allergy in which brown Norway rats are actively sensitized to the allergen ovalbumin and later challenged intranasally with either phosphate-buffered saline solution (vehicle), ovalbumin in vehicle, or ovalbumin and the NO synthase inhibitor N-nitro-L-arginine methyl ester. In each experiment, nasal lavage samples were collected 30, 120, 240, and 360 minutes after challenge. Lavage samples were analyzed for albumin content by ELISA, inflammatory cell concentration with a hemocytometer, and evidence of inflammation by light microscopy. Blocking NO synthesis with N-nitro-L- arginine methyl ester significantly inhibited both albumin exudation and inflammatory cell influx into the nasal cavity during the LPR. These data suggest that NO plays a role in the LPR of nasal allergy.

Original languageEnglish (US)
Pages (from-to)706-711
Number of pages6
JournalOtolaryngology - Head and Neck Surgery
Volume122
Issue number5
StatePublished - 2000
Externally publishedYes

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Nose
Hypersensitivity
Nitric Oxide
Animal Models
Ovalbumin
Histamine
Albumins
Nasal Lavage
Nasal Cavity
Therapeutic Irrigation
Sodium Chloride
Nitric Oxide Synthase
Allergens
Microscopy
Enzyme-Linked Immunosorbent Assay
Phosphates
Inflammation
Light
arginine methyl ester

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Durland, W. F., Lane, A. P., Durland, K. W., Smith, T. L., Johnson, K. L., Prazma, J., & Pillsbury, H. C. (2000). Nitric oxide is a mediator of the late-phase response in an animal model of nasal allergy. Otolaryngology - Head and Neck Surgery, 122(5), 706-711.

Nitric oxide is a mediator of the late-phase response in an animal model of nasal allergy. / Durland, William F.; Lane, Andrew P; Durland, Kimberly W.; Smith, Timothy L.; Johnson, Kenneth L.; Prazma, Jiri; Pillsbury, Harold C.

In: Otolaryngology - Head and Neck Surgery, Vol. 122, No. 5, 2000, p. 706-711.

Research output: Contribution to journalArticle

Durland, WF, Lane, AP, Durland, KW, Smith, TL, Johnson, KL, Prazma, J & Pillsbury, HC 2000, 'Nitric oxide is a mediator of the late-phase response in an animal model of nasal allergy', Otolaryngology - Head and Neck Surgery, vol. 122, no. 5, pp. 706-711.
Durland, William F. ; Lane, Andrew P ; Durland, Kimberly W. ; Smith, Timothy L. ; Johnson, Kenneth L. ; Prazma, Jiri ; Pillsbury, Harold C. / Nitric oxide is a mediator of the late-phase response in an animal model of nasal allergy. In: Otolaryngology - Head and Neck Surgery. 2000 ; Vol. 122, No. 5. pp. 706-711.
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