Nitric oxide inhibits viral replication in murine myocarditis

Charles J. Lowenstein, Susan L. Hill, Anne Lafond-Walker, Jean Wu, Greg Allen, Mike Landavere, Noel R. Rose, Ahvie Herskowitz

Research output: Contribution to journalArticle

Abstract

Nitric oxide (NO) is a radical molecule that not only serves as a vasodilator and neurotransmitter but also acts as a cytotoxic effector molecule of the immune system. The inducible enzyme making NO, inducible NO synthase (iNOS), is transcriptionally activated by IFN-γ and TNF-α, cytokines which are produced during vital infection. We show that iNOS is induced in mice infected with the Coxsackie B3 virus. Macrophages expressing iNOS are identified in the hearts and spleens of infected animals with an antibody raised against iNOS. Infected mice have increased titers of virus and a higher mortality when fed NOS inhibitors. Thus, viral infection induces iNOS in vivo, and NO inhibits viral replication, NO is a novel, nonspecific immune defense against viruses in vivo.

Original languageEnglish (US)
Pages (from-to)1837-1843
Number of pages7
JournalJournal of Clinical Investigation
Volume97
Issue number8
StatePublished - Apr 15 1996

Fingerprint

Myocarditis
Nitric Oxide Synthase
Nitric Oxide
Enterovirus
Nitric Oxide Synthase Type II
Virus Diseases
Viral Load
Vasodilator Agents
Neurotransmitter Agents
Immune System
Spleen
Macrophages
Cytokines
Viruses
Mortality
Antibodies
Enzymes
Infection

Keywords

  • Coxsackievirus
  • cytokines
  • enterovirus
  • inducible nitric oxide synthase
  • macrophage

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lowenstein, C. J., Hill, S. L., Lafond-Walker, A., Wu, J., Allen, G., Landavere, M., ... Herskowitz, A. (1996). Nitric oxide inhibits viral replication in murine myocarditis. Journal of Clinical Investigation, 97(8), 1837-1843.

Nitric oxide inhibits viral replication in murine myocarditis. / Lowenstein, Charles J.; Hill, Susan L.; Lafond-Walker, Anne; Wu, Jean; Allen, Greg; Landavere, Mike; Rose, Noel R.; Herskowitz, Ahvie.

In: Journal of Clinical Investigation, Vol. 97, No. 8, 15.04.1996, p. 1837-1843.

Research output: Contribution to journalArticle

Lowenstein, CJ, Hill, SL, Lafond-Walker, A, Wu, J, Allen, G, Landavere, M, Rose, NR & Herskowitz, A 1996, 'Nitric oxide inhibits viral replication in murine myocarditis', Journal of Clinical Investigation, vol. 97, no. 8, pp. 1837-1843.
Lowenstein CJ, Hill SL, Lafond-Walker A, Wu J, Allen G, Landavere M et al. Nitric oxide inhibits viral replication in murine myocarditis. Journal of Clinical Investigation. 1996 Apr 15;97(8):1837-1843.
Lowenstein, Charles J. ; Hill, Susan L. ; Lafond-Walker, Anne ; Wu, Jean ; Allen, Greg ; Landavere, Mike ; Rose, Noel R. ; Herskowitz, Ahvie. / Nitric oxide inhibits viral replication in murine myocarditis. In: Journal of Clinical Investigation. 1996 ; Vol. 97, No. 8. pp. 1837-1843.
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