Nitric Oxide Inhibits the Rate and Strength of Cardiac Contractions in the Lobster Homarus americanus by Acting on the Cardiac Ganglion

Anand Mahadevan, Jason Lappé, Randall Trenton Rhyne, Nelson D. Cruz-Bermúdez, Eve Marder, Michael F. Goy

Research output: Contribution to journalArticle

Abstract

The lobster heart is synaptically driven by the cardiac ganglion, a spontaneously bursting neural network residing within the cardiac lumen. Here, we present evidence that nitric oxide (NO) plays an inhibitory role in lobster cardiac physiology. (1) NO decreases heartbeat frequency and amplitude. Decreased frequency is a direct consequence of a decreased ganglionic burst rate. Decreased amplitude is an indirect consequence of decreased burst frequency, attributable to the highly facilitating nature of the synapses between cardiac ganglion neurons and muscle fibers (although, during prolonged exposure to NO, amplitude recovers to the original level by a frequency-independent adaptation mechanism). NO does not alter burst duration, spikes per burst, heart muscle contractility, or amplitudes of synaptic potentials evoked by stimulating postganglionic motor nerves. Thus, NO acts on the ganglion, but not on heart muscle. (2) Two observations suggest that NO is produced within the lobster heart. First, immunoblot analysis shows that nitric oxide synthase (NOS) is strongly expressed in heart muscle relative to other muscles. Second, L-nitroarginine (L-NA), an NOS inhibitor, increases the rate of the heartbeat (opposite to the effects of NO). In contrast, the isolated ganglion is insensitive to L-NA, suggesting that heart muscle (but not the ganglion) produces endogenous NO. Basal heart rate varies from animal to animal, and L-NA has the greatest effect on the slowest hearts, presumably because these hearts are producing the most NO. Thus, because the musculature is a site of NOS expression, whereas the ganglion is the only intracardiac target of NO, we hypothesize that NO serves as an inhibitory retrograde transmitter.

Original languageEnglish (US)
Pages (from-to)2813-2824
Number of pages12
JournalJournal of Neuroscience
Volume24
Issue number11
DOIs
StatePublished - Mar 17 2004
Externally publishedYes

Fingerprint

Nephropidae
Ganglia
Nitric Oxide
Nitroarginine
Myocardium
Nitric Oxide Synthase
Myocardial Contraction
Muscles
Synaptic Potentials
Synapses

Keywords

  • Central pattern generator
  • Crustacean
  • Heart
  • Negative chronotropy
  • Negative inotropy
  • Neuromodulation
  • Nitric oxide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nitric Oxide Inhibits the Rate and Strength of Cardiac Contractions in the Lobster Homarus americanus by Acting on the Cardiac Ganglion. / Mahadevan, Anand; Lappé, Jason; Rhyne, Randall Trenton; Cruz-Bermúdez, Nelson D.; Marder, Eve; Goy, Michael F.

In: Journal of Neuroscience, Vol. 24, No. 11, 17.03.2004, p. 2813-2824.

Research output: Contribution to journalArticle

Mahadevan, Anand ; Lappé, Jason ; Rhyne, Randall Trenton ; Cruz-Bermúdez, Nelson D. ; Marder, Eve ; Goy, Michael F. / Nitric Oxide Inhibits the Rate and Strength of Cardiac Contractions in the Lobster Homarus americanus by Acting on the Cardiac Ganglion. In: Journal of Neuroscience. 2004 ; Vol. 24, No. 11. pp. 2813-2824.
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abstract = "The lobster heart is synaptically driven by the cardiac ganglion, a spontaneously bursting neural network residing within the cardiac lumen. Here, we present evidence that nitric oxide (NO) plays an inhibitory role in lobster cardiac physiology. (1) NO decreases heartbeat frequency and amplitude. Decreased frequency is a direct consequence of a decreased ganglionic burst rate. Decreased amplitude is an indirect consequence of decreased burst frequency, attributable to the highly facilitating nature of the synapses between cardiac ganglion neurons and muscle fibers (although, during prolonged exposure to NO, amplitude recovers to the original level by a frequency-independent adaptation mechanism). NO does not alter burst duration, spikes per burst, heart muscle contractility, or amplitudes of synaptic potentials evoked by stimulating postganglionic motor nerves. Thus, NO acts on the ganglion, but not on heart muscle. (2) Two observations suggest that NO is produced within the lobster heart. First, immunoblot analysis shows that nitric oxide synthase (NOS) is strongly expressed in heart muscle relative to other muscles. Second, L-nitroarginine (L-NA), an NOS inhibitor, increases the rate of the heartbeat (opposite to the effects of NO). In contrast, the isolated ganglion is insensitive to L-NA, suggesting that heart muscle (but not the ganglion) produces endogenous NO. Basal heart rate varies from animal to animal, and L-NA has the greatest effect on the slowest hearts, presumably because these hearts are producing the most NO. Thus, because the musculature is a site of NOS expression, whereas the ganglion is the only intracardiac target of NO, we hypothesize that NO serves as an inhibitory retrograde transmitter.",
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