Abstract
Objective: To elucidate the regulation of the nitric oxide (NO) and carbon monoxide (CO) pathways in preeclampsia and to evaluate the ratio of asymmetric dimethylarginine (ADMA) to symmetric dimethylarginine (SDMA) as a marker for preeclampsia. Methods: Maternal plasma and placental samples were obtained from 20 participants with preeclampsia and 23 controls. Enzyme-linked immunosorbent assay was used to measure plasma NO, ADMA, and SDMA as well as placental NO and hemeoxygnase 1 (HO-1). Western blot was used to measure placental dimethylarginine dimethylaminotransferases (DDAH-I and DDAH-II). Results: Placental DDAH-I, placental DDAH-II, placental NO, and placental HO-1 were significantly decreased in participants with preeclampsia. While ADMA and SDMA levels were decreased in preeclampsia, the ADMA-SDMA ratio was not significantly different. Conclusions: Decreased DDAH and HO with preeclampsia suggest that they are important points in the regulatory pathways of NO and CO production that are altered in preeclampsia. The ADMA-SDMA ratio is not a useful test for preeclampsia.
Original language | English (US) |
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Pages (from-to) | 542-548 |
Number of pages | 7 |
Journal | Reproductive Sciences |
Volume | 20 |
Issue number | 5 |
DOIs | |
State | Published - May 2013 |
Externally published | Yes |
Keywords
- asymmetric dimethylarginine
- carbon monoxide
- hemeoxygenase
- nitric oxide
- preeclampsia
ASJC Scopus subject areas
- Obstetrics and Gynecology