Nitric oxide and carbon monoxide production and metabolism in preeclampsia

Robert M. Ehsanipoor, Wilbert Fortson, Laura E. Fitzmaurice, Wu Xiang Liao, Deborah A. Wing, Dong Bao Chen, Kenneth Chan

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To elucidate the regulation of the nitric oxide (NO) and carbon monoxide (CO) pathways in preeclampsia and to evaluate the ratio of asymmetric dimethylarginine (ADMA) to symmetric dimethylarginine (SDMA) as a marker for preeclampsia. Methods: Maternal plasma and placental samples were obtained from 20 participants with preeclampsia and 23 controls. Enzyme-linked immunosorbent assay was used to measure plasma NO, ADMA, and SDMA as well as placental NO and hemeoxygnase 1 (HO-1). Western blot was used to measure placental dimethylarginine dimethylaminotransferases (DDAH-I and DDAH-II). Results: Placental DDAH-I, placental DDAH-II, placental NO, and placental HO-1 were significantly decreased in participants with preeclampsia. While ADMA and SDMA levels were decreased in preeclampsia, the ADMA-SDMA ratio was not significantly different. Conclusions: Decreased DDAH and HO with preeclampsia suggest that they are important points in the regulatory pathways of NO and CO production that are altered in preeclampsia. The ADMA-SDMA ratio is not a useful test for preeclampsia.

Original languageEnglish (US)
Pages (from-to)542-548
Number of pages7
JournalReproductive Sciences
Volume20
Issue number5
DOIs
StatePublished - May 1 2013

Keywords

  • asymmetric dimethylarginine
  • carbon monoxide
  • hemeoxygenase
  • nitric oxide
  • preeclampsia

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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