Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment

Manish Bodas, Colin Van Westphal, Rhett Carpenter-Thompson, Dillip K. Mohanty, Neeraj Vij

Research output: Contribution to journalArticle

Abstract

Waterpipe smoking and e-cigarette vaping, the non-combustible sources of inhaled nicotine exposure are increasingly becoming popular and marketed as safer alternative to cigarette smoking. Hence, this study was designed to investigate the impact of inhaled nicotine exposure on disease causing COPD-emphysema mechanisms. For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5 mM), and/or cysteamine (250 μM, an autophagy inducer and anti-oxidant drug), for 6 hrs. We observed significantly (p

Original languageEnglish (US)
Pages (from-to)441-453
Number of pages13
JournalFree Radical Biology and Medicine
Volume97
DOIs
StatePublished - Aug 1 2016

Keywords

  • Anti-oxidant
  • Autophagy
  • Autophagy-impairment
  • COPD-emphysema
  • Hookah
  • Nicotine
  • Waterpipe
  • Waterpipe-smoke-extract (WPSE)

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

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