Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment

Manish Bodas; Colin Van Westphal; Rhett Carpenter-Thompson; Dillip K. Mohanty; Neeraj Vij

doi:10.1016/j.freeradbiomed.2016.06.017

Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment

Manish Bodas, Colin Van Westphal, Rhett Carpenter-Thompson, Dillip K. Mohanty, Neeraj Vij

School of Medicine

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Waterpipe smoking and e-cigarette vaping, the non-combustible sources of inhaled nicotine exposure are increasingly becoming popular and marketed as safer alternative to cigarette smoking. Hence, this study was designed to investigate the impact of inhaled nicotine exposure on disease causing COPD-emphysema mechanisms. For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5 mM), and/or cysteamine (250 μM, an autophagy inducer and anti-oxidant drug), for 6 hrs. We observed significantly (p

Original language	English (US)
Pages (from-to)	441-453
Number of pages	13
Journal	Free Radical Biology and Medicine
Volume	97
DOIs	https://doi.org/10.1016/j.freeradbiomed.2016.06.017
State	Published - Aug 1 2016

Keywords

Anti-oxidant
Autophagy
Autophagy-impairment
COPD-emphysema
Hookah
Nicotine
Waterpipe
Waterpipe-smoke-extract (WPSE)

ASJC Scopus subject areas

Biochemistry
Physiology (medical)

Access to Document

10.1016/j.freeradbiomed.2016.06.017

Cite this

@article{9e3941d52e1f4608b7e2b96663d56172,

title = "Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment",

abstract = "Waterpipe smoking and e-cigarette vaping, the non-combustible sources of inhaled nicotine exposure are increasingly becoming popular and marketed as safer alternative to cigarette smoking. Hence, this study was designed to investigate the impact of inhaled nicotine exposure on disease causing COPD-emphysema mechanisms. For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5 mM), and/or cysteamine (250 μM, an autophagy inducer and anti-oxidant drug), for 6 hrs. We observed significantly (p",

keywords = "Anti-oxidant, Autophagy, Autophagy-impairment, COPD-emphysema, Hookah, Nicotine, Waterpipe, Waterpipe-smoke-extract (WPSE)",

author = "Manish Bodas and {Van Westphal}, Colin and Rhett Carpenter-Thompson and {K. Mohanty}, Dillip and Neeraj Vij",

year = "2016",

month = aug,

day = "1",

doi = "10.1016/j.freeradbiomed.2016.06.017",

language = "English (US)",

volume = "97",

pages = "441--453",

journal = "Free Radical Biology and Medicine",

issn = "0891-5849",

publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment

AU - Bodas, Manish

AU - Van Westphal, Colin

AU - Carpenter-Thompson, Rhett

AU - K. Mohanty, Dillip

AU - Vij, Neeraj

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Waterpipe smoking and e-cigarette vaping, the non-combustible sources of inhaled nicotine exposure are increasingly becoming popular and marketed as safer alternative to cigarette smoking. Hence, this study was designed to investigate the impact of inhaled nicotine exposure on disease causing COPD-emphysema mechanisms. For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5 mM), and/or cysteamine (250 μM, an autophagy inducer and anti-oxidant drug), for 6 hrs. We observed significantly (p

AB - Waterpipe smoking and e-cigarette vaping, the non-combustible sources of inhaled nicotine exposure are increasingly becoming popular and marketed as safer alternative to cigarette smoking. Hence, this study was designed to investigate the impact of inhaled nicotine exposure on disease causing COPD-emphysema mechanisms. For in vitro studies, human bronchial epithelial cells (Beas2b) were treated with waterpipe smoke extract (WPSE, 5%), nicotine (5 mM), and/or cysteamine (250 μM, an autophagy inducer and anti-oxidant drug), for 6 hrs. We observed significantly (p

KW - Anti-oxidant

KW - Autophagy

KW - Autophagy-impairment

KW - COPD-emphysema

KW - Hookah

KW - Nicotine

KW - Waterpipe

KW - Waterpipe-smoke-extract (WPSE)

UR - http://www.scopus.com/inward/record.url?scp=84978882359&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978882359&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2016.06.017

DO - 10.1016/j.freeradbiomed.2016.06.017

M3 - Article

C2 - 27394171

AN - SCOPUS:84978882359

SN - 0891-5849

VL - 97

SP - 441

EP - 453

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

ER -

Nicotine exposure induces bronchial epithelial cell apoptosis and senescence via ROS mediated autophagy-impairment

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this