NHE2 is the major brush border NA/H exchanger isoform in the avian colon and is stimulated by mineralocorticoids

Mark Donowitz, C. De La Horra, I. S. Wood, J. Dyer, S. M. Gribble, Chung Ming Tse, S. P. Shirazi-Beechey, A. A. Ilundain

Research output: Contribution to journalArticle

Abstract

We previously reported that mammalian small intestinal and colonic brush borders contained both epithelial Na/H exchangers NHE2 and NHE3 Now we show that in the avian colon (chicken) NHE2 is the major functional isoform under basal conditions and when stimulated with mineralocorticoids. Hubbard chickens were maintained for 2 weeks on a high (1 gm per day) or low (0) NaCl diet. After sacrifice, the colons were removed and BB prepared by Mg precipitation and 22Na uptake and D-[14C] glucose uptake determined in the BB vesicles NHE2 and NHR3 were separated by differential sensitivity to HOE694 (NHE2 defined as Na/H exchange inhibited by 50 uM HOE694). Low Na diet increased plasma aldosterone from 10 to 207 pg/ml. On the high NaCl diet both NHE2 and NHE3 contributed to Nail exchange but the majority was due to NHE2 (86%) Low NaCl diet significantly decreased colonic Na dependent glucose uptake and increased colonic BB Na/H exchange (342 vs 190 pmol/mg protein/5 min), the increase in BB Na/H exchange was entirely due to an increase in NHE2 (319 vs 163) with no change in NHE3 activity, (23 vs 27) NHE2 and NHE3 were present in colonic BB but not BEM immunocytochemistry, and Western analysis showed that low Na diet increased the amount of NHE2 in the colonic BB. Summary 1) NHE2 and NHE3 are both present in the BB and not BLM of chicken colonocytes; 2) NHE2 is the major physiologic colonic BB Na/H exchanger under basal conditions; 3) increasing aldosterone increases colonic BB Na/H exchange and decreases Na dependent glucose uptake; 4) the aldosterone stimulation of colonic brush border Na/H exchange is due to increased activity and amount of NHE2.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

mineralocorticoids
Mineralocorticoids
Brushes
Nutrition
Microvilli
microvilli
colon
Protein Isoforms
Colon
aldosterone
Diet
Aldosterone
uptake mechanisms
Chickens
Sodium-Hydrogen Antiporter
diet
chickens
Glucose
glucose
Hubbard (chicken breed)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

NHE2 is the major brush border NA/H exchanger isoform in the avian colon and is stimulated by mineralocorticoids. / Donowitz, Mark; De La Horra, C.; Wood, I. S.; Dyer, J.; Gribble, S. M.; Tse, Chung Ming; Shirazi-Beechey, S. P.; Ilundain, A. A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Donowitz, M, De La Horra, C, Wood, IS, Dyer, J, Gribble, SM, Tse, CM, Shirazi-Beechey, SP & Ilundain, AA 1997, 'NHE2 is the major brush border NA/H exchanger isoform in the avian colon and is stimulated by mineralocorticoids', FASEB Journal, vol. 11, no. 3.
Donowitz, Mark ; De La Horra, C. ; Wood, I. S. ; Dyer, J. ; Gribble, S. M. ; Tse, Chung Ming ; Shirazi-Beechey, S. P. ; Ilundain, A. A. / NHE2 is the major brush border NA/H exchanger isoform in the avian colon and is stimulated by mineralocorticoids. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
@article{42d5904f4fe34f19a2e86ec0924e3705,
title = "NHE2 is the major brush border NA/H exchanger isoform in the avian colon and is stimulated by mineralocorticoids",
abstract = "We previously reported that mammalian small intestinal and colonic brush borders contained both epithelial Na/H exchangers NHE2 and NHE3 Now we show that in the avian colon (chicken) NHE2 is the major functional isoform under basal conditions and when stimulated with mineralocorticoids. Hubbard chickens were maintained for 2 weeks on a high (1 gm per day) or low (0) NaCl diet. After sacrifice, the colons were removed and BB prepared by Mg precipitation and 22Na uptake and D-[14C] glucose uptake determined in the BB vesicles NHE2 and NHR3 were separated by differential sensitivity to HOE694 (NHE2 defined as Na/H exchange inhibited by 50 uM HOE694). Low Na diet increased plasma aldosterone from 10 to 207 pg/ml. On the high NaCl diet both NHE2 and NHE3 contributed to Nail exchange but the majority was due to NHE2 (86{\%}) Low NaCl diet significantly decreased colonic Na dependent glucose uptake and increased colonic BB Na/H exchange (342 vs 190 pmol/mg protein/5 min), the increase in BB Na/H exchange was entirely due to an increase in NHE2 (319 vs 163) with no change in NHE3 activity, (23 vs 27) NHE2 and NHE3 were present in colonic BB but not BEM immunocytochemistry, and Western analysis showed that low Na diet increased the amount of NHE2 in the colonic BB. Summary 1) NHE2 and NHE3 are both present in the BB and not BLM of chicken colonocytes; 2) NHE2 is the major physiologic colonic BB Na/H exchanger under basal conditions; 3) increasing aldosterone increases colonic BB Na/H exchange and decreases Na dependent glucose uptake; 4) the aldosterone stimulation of colonic brush border Na/H exchange is due to increased activity and amount of NHE2.",
author = "Mark Donowitz and {De La Horra}, C. and Wood, {I. S.} and J. Dyer and Gribble, {S. M.} and Tse, {Chung Ming} and Shirazi-Beechey, {S. P.} and Ilundain, {A. A.}",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "3",

}

TY - JOUR

T1 - NHE2 is the major brush border NA/H exchanger isoform in the avian colon and is stimulated by mineralocorticoids

AU - Donowitz, Mark

AU - De La Horra, C.

AU - Wood, I. S.

AU - Dyer, J.

AU - Gribble, S. M.

AU - Tse, Chung Ming

AU - Shirazi-Beechey, S. P.

AU - Ilundain, A. A.

PY - 1997

Y1 - 1997

N2 - We previously reported that mammalian small intestinal and colonic brush borders contained both epithelial Na/H exchangers NHE2 and NHE3 Now we show that in the avian colon (chicken) NHE2 is the major functional isoform under basal conditions and when stimulated with mineralocorticoids. Hubbard chickens were maintained for 2 weeks on a high (1 gm per day) or low (0) NaCl diet. After sacrifice, the colons were removed and BB prepared by Mg precipitation and 22Na uptake and D-[14C] glucose uptake determined in the BB vesicles NHE2 and NHR3 were separated by differential sensitivity to HOE694 (NHE2 defined as Na/H exchange inhibited by 50 uM HOE694). Low Na diet increased plasma aldosterone from 10 to 207 pg/ml. On the high NaCl diet both NHE2 and NHE3 contributed to Nail exchange but the majority was due to NHE2 (86%) Low NaCl diet significantly decreased colonic Na dependent glucose uptake and increased colonic BB Na/H exchange (342 vs 190 pmol/mg protein/5 min), the increase in BB Na/H exchange was entirely due to an increase in NHE2 (319 vs 163) with no change in NHE3 activity, (23 vs 27) NHE2 and NHE3 were present in colonic BB but not BEM immunocytochemistry, and Western analysis showed that low Na diet increased the amount of NHE2 in the colonic BB. Summary 1) NHE2 and NHE3 are both present in the BB and not BLM of chicken colonocytes; 2) NHE2 is the major physiologic colonic BB Na/H exchanger under basal conditions; 3) increasing aldosterone increases colonic BB Na/H exchange and decreases Na dependent glucose uptake; 4) the aldosterone stimulation of colonic brush border Na/H exchange is due to increased activity and amount of NHE2.

AB - We previously reported that mammalian small intestinal and colonic brush borders contained both epithelial Na/H exchangers NHE2 and NHE3 Now we show that in the avian colon (chicken) NHE2 is the major functional isoform under basal conditions and when stimulated with mineralocorticoids. Hubbard chickens were maintained for 2 weeks on a high (1 gm per day) or low (0) NaCl diet. After sacrifice, the colons were removed and BB prepared by Mg precipitation and 22Na uptake and D-[14C] glucose uptake determined in the BB vesicles NHE2 and NHR3 were separated by differential sensitivity to HOE694 (NHE2 defined as Na/H exchange inhibited by 50 uM HOE694). Low Na diet increased plasma aldosterone from 10 to 207 pg/ml. On the high NaCl diet both NHE2 and NHE3 contributed to Nail exchange but the majority was due to NHE2 (86%) Low NaCl diet significantly decreased colonic Na dependent glucose uptake and increased colonic BB Na/H exchange (342 vs 190 pmol/mg protein/5 min), the increase in BB Na/H exchange was entirely due to an increase in NHE2 (319 vs 163) with no change in NHE3 activity, (23 vs 27) NHE2 and NHE3 were present in colonic BB but not BEM immunocytochemistry, and Western analysis showed that low Na diet increased the amount of NHE2 in the colonic BB. Summary 1) NHE2 and NHE3 are both present in the BB and not BLM of chicken colonocytes; 2) NHE2 is the major physiologic colonic BB Na/H exchanger under basal conditions; 3) increasing aldosterone increases colonic BB Na/H exchange and decreases Na dependent glucose uptake; 4) the aldosterone stimulation of colonic brush border Na/H exchange is due to increased activity and amount of NHE2.

UR - http://www.scopus.com/inward/record.url?scp=33750236725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750236725&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33750236725

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 3

ER -