NHE2 and NHE3 are human and rabbit intestinal brush-border proteins

W. Alexandra Hoogerwerf, Su C. Tsao, Olivier Devuyst, Susan A. Levine, C. H.Chris Yun, Jeannie W. Yip, Michael E. Cohen, Patricia D. Wilson, Audrey J. Lazenby, Chung Ming Tse, Maek Donowitz

Research output: Contribution to journalArticlepeer-review


Rabbit NHE2 and NHE3 are two epithelial isoform Na+/H+ exchangers (NHE), the messages for which are found predominantly and entirely, respectively, in renal, intestinal, and gastric mucosa. The current studies used Western analysis and immunohistochemistry to identify and characterize the apical vs. baselateral membrane distribution of NHE2 and NHE3 in intestinal epithelial cells. Based on Western analysis, NHE2 and NHE3 both are present in brush- border but not basolateral membranes of small intestine. Both NHE2 and NHE3 are 85-kDa proteins. Consistent with Western analysis, NHE2 and NHE3 are immunolocalized to the brush-border but not basolateral membranes of villus epithelial cells, but not goblet cells, in human jejunum and ileum and in surface epithelial cells in the ascending and descending colon and rectum. In addition, NHE2 and NHE3 are present in small amounts in the crypt cell brush border of human jejunum, ileum, ascending and descending colon, and rectum. In rabbit jejunum, ileum, and ascending colon, NHE2 and NHE3 are present in the brush border of epithelial and not goblet cells, again much more in the villus (small intestine)/surface cells (colon) than the crypt. NHE2 but not NHE3 is present in the brush border of rabbit descending colon surface cells and in small amounts in crypt cells. NHE2 and NHE3 are both human and rabbit small intestinal and colonic epithelial cell brush-border Na+/H+ exchanger isoforms that colocalize in all intestinal segments except rabbit descending colon, which lacks NHE3.

Original languageEnglish (US)
Pages (from-to)G29-G41
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number1 33-1
StatePublished - 1996


  • colon
  • immunohistochemistry
  • small intestine
  • sodium absorption
  • sodium/hydrogen exchanger

ASJC Scopus subject areas

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)


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