NGF-TrkA Signaling by Sensory Nerves Coordinates the Vascularization and Ossification of Developing Endochondral Bone

Ryan E. Tomlinson, Zhi Li, Qian Zhang, Brian C. Goh, Zhu Li, Daniel L J Thorek, Labchan Rajbhandari, Thomas M. Brushart, Liliana Minichiello, Fengquan Zhou, Arun Venkatesan, Thomas L. Clemens

Research output: Research - peer-reviewArticle

Abstract

Developing tissues dictate the amount and type of innervation they require by secreting neurotrophins, which promote neuronal survival by activating distinct tyrosine kinase receptors. Here, we show that nerve growth factor (NGF) signaling through neurotrophic tyrosine kinase receptor type 1 (TrkA) directs innervation of the developing mouse femur to promote vascularization and osteoprogenitor lineage progression. At the start of primary ossification, TrkA-positive axons were observed at perichondrial bone surfaces, coincident with NGF expression in cells adjacent to centers of incipient ossification. Inactivation of TrkA signaling during embryogenesis in TrkAF592A mice impaired innervation, delayed vascular invasion of the primary and secondary ossification centers, decreased numbers of Osx-expressing osteoprogenitors, and decreased femoral length and volume. These same phenotypic abnormalities were observed in mice following tamoxifen-induced disruption of NGF in Col2-expressing perichondrial osteochondral progenitors. We conclude that NGF serves as a skeletal neurotrophin to promote sensory innervation of developing long bones, a process critical for normal primary and secondary ossification.

LanguageEnglish (US)
Pages2723-2735
Number of pages13
JournalCell Reports
Volume16
Issue number10
DOIs
StatePublished - Sep 1 2016

Fingerprint

Nerve Growth Factor
Osteogenesis
Bone and Bones
Bone
Nerve Growth Factors
trkA Receptor
Receptor Protein-Tyrosine Kinases
Tamoxifen
Thigh
Femur
Embryonic Development
Blood Vessels
Axons
Tissue

Keywords

  • endochondral bone
  • nerve growth factor
  • neurotrophic tyrosine kinase receptor type 1
  • sensory nerves

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

NGF-TrkA Signaling by Sensory Nerves Coordinates the Vascularization and Ossification of Developing Endochondral Bone. / Tomlinson, Ryan E.; Li, Zhi; Zhang, Qian; Goh, Brian C.; Li, Zhu; Thorek, Daniel L J; Rajbhandari, Labchan; Brushart, Thomas M.; Minichiello, Liliana; Zhou, Fengquan; Venkatesan, Arun; Clemens, Thomas L.

In: Cell Reports, Vol. 16, No. 10, 01.09.2016, p. 2723-2735.

Research output: Research - peer-reviewArticle

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