NF-κB-mediated activation of the human immunodeficiency virus enhancer

Site of transcriptional initiation is independent of the TATA box

A. Bielinska, S. Krasnow, G. J. Nabel

Research output: Contribution to journalArticle

Abstract

The activation of the human immunodeficiency virus (HIV) enhancer in T cells can occur through multiple independent pathways. This enhancer is stimulated by NF-κB or through alternative mechanisms, including E1A transactivation, which is dependent on the TATA box. In this report, the role of the TATA box in activation by NF-κB is examined. E1A stimulation of the HIV enhancer requires the presence of the TATA sequence in the sense orientation. Analysis of mutant HIV enhancer plasmids shows that basal mRNA levels are reduced when the TATA sequence is altered but that inducibility of NF-κB and the site of transcriptional initiation are unchanged. These data suggest that transcriptional initiation in this class II promoter is determined by an initiator factor which does not require binding to the TATA sequence. Because κB is found in a variety of viruses, this mechanism may be relevant to the activation of other viral enhancers.

Original languageEnglish (US)
Pages (from-to)4097-4100
Number of pages4
JournalJournal of Virology
Volume63
Issue number9
StatePublished - 1989
Externally publishedYes

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TATA box
TATA Box
Human immunodeficiency virus
HIV
Virus Activation
transcriptional activation
Transcriptional Activation
plasmids
Plasmids
T-lymphocytes
promoter regions
Viruses
T-Lymphocytes
viruses
mutants
Messenger RNA

ASJC Scopus subject areas

  • Immunology

Cite this

NF-κB-mediated activation of the human immunodeficiency virus enhancer : Site of transcriptional initiation is independent of the TATA box. / Bielinska, A.; Krasnow, S.; Nabel, G. J.

In: Journal of Virology, Vol. 63, No. 9, 1989, p. 4097-4100.

Research output: Contribution to journalArticle

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