Newly synthesized acetylcholine receptors are located in the Golgi apparatus

D. M. Fambrough, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]α-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated α-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface ACh receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2h.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalJournal of Cell Biology
Volume76
Issue number1
StatePublished - 1978
Externally publishedYes

Fingerprint

Golgi Apparatus
Cholinergic Receptors
Bungarotoxins
Saponins
Muscle Cells
Skeletal Muscle
Cell Membrane
Electrons
Population

ASJC Scopus subject areas

  • Cell Biology

Cite this

Newly synthesized acetylcholine receptors are located in the Golgi apparatus. / Fambrough, D. M.; Devreotes, Peter N.

In: Journal of Cell Biology, Vol. 76, No. 1, 1978, p. 237-244.

Research output: Contribution to journalArticle

@article{b38c70c58d554c3291456285874e4b6d,
title = "Newly synthesized acetylcholine receptors are located in the Golgi apparatus",
abstract = "Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]α-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated α-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface ACh receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2h.",
author = "Fambrough, {D. M.} and Devreotes, {Peter N}",
year = "1978",
language = "English (US)",
volume = "76",
pages = "237--244",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

TY - JOUR

T1 - Newly synthesized acetylcholine receptors are located in the Golgi apparatus

AU - Fambrough, D. M.

AU - Devreotes, Peter N

PY - 1978

Y1 - 1978

N2 - Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]α-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated α-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface ACh receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2h.

AB - Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]α-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated α-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface ACh receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2h.

UR - http://www.scopus.com/inward/record.url?scp=0017884641&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017884641&partnerID=8YFLogxK

M3 - Article

VL - 76

SP - 237

EP - 244

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -