Newly synthesized acetylcholine receptors are located in the Golgi apparatus

D. M. Fambrough, P. N. Devreotes

Research output: Contribution to journalArticle

Abstract

Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]α-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated α-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface ACh receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2h.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalJournal of Cell Biology
Volume76
Issue number1
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • Cell Biology

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