New vectors for direct cloning of PCR products

Jooyeun Cha; William Bishai; Srinivasan Chandrasegaran

doi:10.1016/0378-1119(93)90498-R

New vectors for direct cloning of PCR products

Jooyeun Cha, William Bishai, Srinivasan Chandrasegaran

Johns Hopkins Hospital

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

Original language	English (US)
Pages (from-to)	369-370
Number of pages	2
Journal	Gene
Volume	136
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-1119(93)90498-R
State	Published - Dec 22 1993

Keywords

Recombinant DNA
T/A cloning vectors
phage M13mp

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(93)90498-R

Cite this

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title = "New vectors for direct cloning of PCR products",

abstract = "We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.",

keywords = "Recombinant DNA, T/A cloning vectors, phage M13mp",

author = "Jooyeun Cha and William Bishai and Srinivasan Chandrasegaran",

note = "Funding Information: We thankP rofessorH amilton 0. Smithf or helpfuls ug-gestionsT. his work was supportedb y NIH Grant GM 42140.S .C. has a Faculty ResearchA ward (FRA 65569) from the AmericanC ancerS ociety.W .B. has a Postdoctoral ResearchF ellowshipf or Physiciansf rom Howard HughesM edicalI nstitute.W e thankt he EHS Centerc ore facility (supportedb y Grant ES 03819)f or synthesiso f oligos.",

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doi = "10.1016/0378-1119(93)90498-R",

language = "English (US)",

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T1 - New vectors for direct cloning of PCR products

AU - Cha, Jooyeun

AU - Bishai, William

AU - Chandrasegaran, Srinivasan

N1 - Funding Information: We thankP rofessorH amilton 0. Smithf or helpfuls ug-gestionsT. his work was supportedb y NIH Grant GM 42140.S .C. has a Faculty ResearchA ward (FRA 65569) from the AmericanC ancerS ociety.W .B. has a Postdoctoral ResearchF ellowshipf or Physiciansf rom Howard HughesM edicalI nstitute.W e thankt he EHS Centerc ore facility (supportedb y Grant ES 03819)f or synthesiso f oligos.

PY - 1993/12/22

Y1 - 1993/12/22

N2 - We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

AB - We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

KW - Recombinant DNA

KW - T/A cloning vectors

KW - phage M13mp

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DO - 10.1016/0378-1119(93)90498-R

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SN - 0378-1119

VL - 136

SP - 369

EP - 370

JO - Gene

JF - Gene

IS - 1-2

ER -

New vectors for direct cloning of PCR products

Abstract

Keywords

ASJC Scopus subject areas

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