New vectors for direct cloning of PCR products

Research output: Contribution to journalArticle

Abstract

We describe the construction of two new vectors for direct cloning of polymerase chain reaction (PCR) products. This was done by inserting a synthetic DNA fragment containing two adjacent XcmI sites between the Asp718 and BamHI sites of the M13mp18 and M13mp19 phages. Cleavage of these M13 derivatives with XcmI will result in a linearized vector with a single thymidine nucleotide at the 3' ends. Thus, these vectors would be very useful for direct cloning of PCR-generated products with high efficiency.

Original languageEnglish (US)
Pages (from-to)369-370
Number of pages2
JournalGene
Volume136
Issue number1-2
DOIs
StatePublished - Dec 22 1993

Fingerprint

Genetic Vectors
Polymerase Chain Reaction
Bacteriophages
Thymidine
Organism Cloning
Nucleotides
DNA

Keywords

  • phage M13mp
  • Recombinant DNA
  • T/A cloning vectors

ASJC Scopus subject areas

  • Genetics

Cite this

New vectors for direct cloning of PCR products. / Cha, Jooyeun; Bishai, William Ramses; Chandrasegaran, Srinivasan.

In: Gene, Vol. 136, No. 1-2, 22.12.1993, p. 369-370.

Research output: Contribution to journalArticle

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