New techniques for in vitro culture of human retinal pigment epithelium

M. A. Del Monte, I. H. Maumenee

Research output: Contribution to journalArticle

Abstract

The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.

Original languageEnglish (US)
Pages (from-to)327-338
Number of pages12
JournalBirth Defects: Original Article Series
Volume16
Issue number2
StatePublished - 1980

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Retinal Pigment Epithelium
Culture Techniques
Pigmentation
Epidermal Growth Factor
Collagen
Epithelium
In Vitro Techniques

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics(clinical)

Cite this

New techniques for in vitro culture of human retinal pigment epithelium. / Del Monte, M. A.; Maumenee, I. H.

In: Birth Defects: Original Article Series, Vol. 16, No. 2, 1980, p. 327-338.

Research output: Contribution to journalArticle

Del Monte, M. A. ; Maumenee, I. H. / New techniques for in vitro culture of human retinal pigment epithelium. In: Birth Defects: Original Article Series. 1980 ; Vol. 16, No. 2. pp. 327-338.
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