Abstract
The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 327-338 |
| Number of pages | 12 |
| Journal | Birth Defects: Original Article Series |
| Volume | 16 |
| Issue number | 2 |
| State | Published - 1980 |
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ASJC Scopus subject areas
- Developmental Biology
- Genetics(clinical)
Cite this
New techniques for in vitro culture of human retinal pigment epithelium. / Del Monte, M. A.; Maumenee, I. H.
In: Birth Defects: Original Article Series, Vol. 16, No. 2, 1980, p. 327-338.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - New techniques for in vitro culture of human retinal pigment epithelium
AU - Del Monte, M. A.
AU - Maumenee, I. H.
PY - 1980
Y1 - 1980
N2 - The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.
AB - The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019231717&partnerID=8YFLogxK
M3 - Article
C2 - 7011436
AN - SCOPUS:0019231717
VL - 16
SP - 327
EP - 338
JO - Birth Defects: Original Article Series
JF - Birth Defects: Original Article Series
SN - 0547-6844
IS - 2
ER -