New techniques for in vitro culture of human retinal pigment epithelium

M. A. Del Monte; I. H. Maumenee

New techniques for in vitro culture of human retinal pigment epithelium

M. A. Del Monte, I. H. Maumenee

University Administration

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.

Original language	English (US)
Pages (from-to)	327-338
Number of pages	12
Journal	Birth Defects: Original Article Series
Volume	16
Issue number	2
State	Published - Jan 1 1980

ASJC Scopus subject areas

Developmental Biology
Genetics(clinical)

Cite this

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abstract = "The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.",

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AB - The proper functioning of the many types of ocular epithelium, most notably the RPE, is critical to the sensory functions of the human eye. We have developed new techniques for the in vitro culture of human RPE using collagen coated vessels and EGF. These techniques increased culture success from 14% to 76% of pairs of eyes. Human RPE has been cultured for up to 4 months while retaining marked pigmentation and epithelioid characteristics. Use of pure cultures of human RPE will allow biochemical study of this important tissue.

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New techniques for in vitro culture of human retinal pigment epithelium

Abstract

ASJC Scopus subject areas

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