New parameters in assessment of human donor corneal stroma

Marie Borderie, Kate Grieve, Kristina Irsch, Djida Ghoubay, Cristina Georgeon, Celine De Sousa, Laurent Laroche, Vincent M. Borderie

Research output: Contribution to journalArticle

Abstract

Purpose: To provide quantitative parameters for assessment of human donor corneal stroma by imaging stromal features of diseased and normal human corneas with full-field optical coherence microscopy (FFOCM), using confocal microscopy (CM) and histology as reference techniques. Methods: Bowman's layer (BL) thickness and keratocyte density were assessed ex vivo in 23 human donor corneas and 27 human pathological corneas (keratoconus and other corneal disorders) with FFOCM, CM and histology. Stromal backscattering was assessed with FFOCM. Additionally, 10 normal human corneas were assessed in vivo with CM. Results: In FFOCM, the logarithm of the normalized stromal reflectivity was a linear function of stromal depth (R2 = 0.95) in human donor corneas. Compared with keratoconus corneas, human donor corneas featured higher BL thickness (p = 0.0014) with lower coefficient of variation (BL-COV; p = 0.0002), and linear logarithmic stromal reflectivity with depth (higher R2, p = 0.0001). Compared with other corneal disorders, human donor corneas featured lower BL-COV (p = 0.012) and higher R2 (p = 0.0001). Using the 95% confidence limits of the human donor cornea group, BL thickness < 6.5 μm (sensitivity, 57%; specificity, 100%), BL-COV > 18.6% (79%; 100%) and R2 < 0.94 (93%; 71%) were revealed as indictors of abnormal cornea. In CM, keratocyte density decreased with stromal depth (r = -0.56). The mean overall keratocyte density (cells/mm2) was 205 in human donor corneas, 244 in keratoconus, 176 in other corneal disorders and 386 in normal corneas. Conclusion: Full-field optical coherence microscopy (FFOCM) provides precise and reliable parameters for non-invasive assessment of human donor corneal stroma during storage, enabling detection of stromal disorders that could impair the results of keratoplasty.

Original languageEnglish (US)
JournalActa Ophthalmologica
DOIs
StateAccepted/In press - 2017

Fingerprint

Corneal Stroma
Cornea
Microscopy
Confocal Microscopy
Keratoconus
Histology
Corneal Transplantation

Keywords

  • Cornea
  • Donor tissue
  • Keratocyte
  • Optical coherence tomography
  • Stroma

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Borderie, M., Grieve, K., Irsch, K., Ghoubay, D., Georgeon, C., De Sousa, C., ... Borderie, V. M. (Accepted/In press). New parameters in assessment of human donor corneal stroma. Acta Ophthalmologica. https://doi.org/10.1111/aos.13351

New parameters in assessment of human donor corneal stroma. / Borderie, Marie; Grieve, Kate; Irsch, Kristina; Ghoubay, Djida; Georgeon, Cristina; De Sousa, Celine; Laroche, Laurent; Borderie, Vincent M.

In: Acta Ophthalmologica, 2017.

Research output: Contribution to journalArticle

Borderie, M, Grieve, K, Irsch, K, Ghoubay, D, Georgeon, C, De Sousa, C, Laroche, L & Borderie, VM 2017, 'New parameters in assessment of human donor corneal stroma', Acta Ophthalmologica. https://doi.org/10.1111/aos.13351
Borderie, Marie ; Grieve, Kate ; Irsch, Kristina ; Ghoubay, Djida ; Georgeon, Cristina ; De Sousa, Celine ; Laroche, Laurent ; Borderie, Vincent M. / New parameters in assessment of human donor corneal stroma. In: Acta Ophthalmologica. 2017.
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AU - Borderie, Marie

AU - Grieve, Kate

AU - Irsch, Kristina

AU - Ghoubay, Djida

AU - Georgeon, Cristina

AU - De Sousa, Celine

AU - Laroche, Laurent

AU - Borderie, Vincent M.

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N2 - Purpose: To provide quantitative parameters for assessment of human donor corneal stroma by imaging stromal features of diseased and normal human corneas with full-field optical coherence microscopy (FFOCM), using confocal microscopy (CM) and histology as reference techniques. Methods: Bowman's layer (BL) thickness and keratocyte density were assessed ex vivo in 23 human donor corneas and 27 human pathological corneas (keratoconus and other corneal disorders) with FFOCM, CM and histology. Stromal backscattering was assessed with FFOCM. Additionally, 10 normal human corneas were assessed in vivo with CM. Results: In FFOCM, the logarithm of the normalized stromal reflectivity was a linear function of stromal depth (R2 = 0.95) in human donor corneas. Compared with keratoconus corneas, human donor corneas featured higher BL thickness (p = 0.0014) with lower coefficient of variation (BL-COV; p = 0.0002), and linear logarithmic stromal reflectivity with depth (higher R2, p = 0.0001). Compared with other corneal disorders, human donor corneas featured lower BL-COV (p = 0.012) and higher R2 (p = 0.0001). Using the 95% confidence limits of the human donor cornea group, BL thickness < 6.5 μm (sensitivity, 57%; specificity, 100%), BL-COV > 18.6% (79%; 100%) and R2 < 0.94 (93%; 71%) were revealed as indictors of abnormal cornea. In CM, keratocyte density decreased with stromal depth (r = -0.56). The mean overall keratocyte density (cells/mm2) was 205 in human donor corneas, 244 in keratoconus, 176 in other corneal disorders and 386 in normal corneas. Conclusion: Full-field optical coherence microscopy (FFOCM) provides precise and reliable parameters for non-invasive assessment of human donor corneal stroma during storage, enabling detection of stromal disorders that could impair the results of keratoplasty.

AB - Purpose: To provide quantitative parameters for assessment of human donor corneal stroma by imaging stromal features of diseased and normal human corneas with full-field optical coherence microscopy (FFOCM), using confocal microscopy (CM) and histology as reference techniques. Methods: Bowman's layer (BL) thickness and keratocyte density were assessed ex vivo in 23 human donor corneas and 27 human pathological corneas (keratoconus and other corneal disorders) with FFOCM, CM and histology. Stromal backscattering was assessed with FFOCM. Additionally, 10 normal human corneas were assessed in vivo with CM. Results: In FFOCM, the logarithm of the normalized stromal reflectivity was a linear function of stromal depth (R2 = 0.95) in human donor corneas. Compared with keratoconus corneas, human donor corneas featured higher BL thickness (p = 0.0014) with lower coefficient of variation (BL-COV; p = 0.0002), and linear logarithmic stromal reflectivity with depth (higher R2, p = 0.0001). Compared with other corneal disorders, human donor corneas featured lower BL-COV (p = 0.012) and higher R2 (p = 0.0001). Using the 95% confidence limits of the human donor cornea group, BL thickness < 6.5 μm (sensitivity, 57%; specificity, 100%), BL-COV > 18.6% (79%; 100%) and R2 < 0.94 (93%; 71%) were revealed as indictors of abnormal cornea. In CM, keratocyte density decreased with stromal depth (r = -0.56). The mean overall keratocyte density (cells/mm2) was 205 in human donor corneas, 244 in keratoconus, 176 in other corneal disorders and 386 in normal corneas. Conclusion: Full-field optical coherence microscopy (FFOCM) provides precise and reliable parameters for non-invasive assessment of human donor corneal stroma during storage, enabling detection of stromal disorders that could impair the results of keratoplasty.

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KW - Donor tissue

KW - Keratocyte

KW - Optical coherence tomography

KW - Stroma

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