New insights provided by a comparison of impaired deformability with erythrocyte oxidative stress for sickle cell disease

Viachaslau Barodka, Nagababu Enika, Joy G. Mohanty, Daniel Nyhan, Dan E Berkowitz, Joseph M. Rifkind, John Strouse

Research output: Contribution to journalArticle

Abstract

Sickle cell disease (SCD) is associated with increase in oxidative stress and irreversible membrane changes that originates from the instability and polymerization of deoxygenated hemoglobin S (HbS). The relationship between erythrocyte membrane changes as assessed by a decrease in deformability and oxidative stress as assessed by an increase in heme degradation was investigated. The erythrocyte deformability and heme degradation for 27 subjects with SCD and 7 with sickle trait were compared with normal healthy adults. Changes in both deformability and heme degradation increased in the order of control to trait to non-crisis SCD to crisis SCD resulting in a very significantly negative correlation between deformability and heme degradation. However, a quantitative analysis of the changes in deformability and heme degradation for these different groups of subjects indicated that sickle trait had a much smaller effect on deformability than on heme degradation, while crisis affects deformability to a greater extent than heme degradation. These findings provide insights into the relative contributions of erythrocyte oxidative stress and membrane damage during the progression of SCD providing a better understanding of the pathophysiology of SCD.

Original languageEnglish (US)
Pages (from-to)230-235
Number of pages6
JournalBlood Cells, Molecules, and Diseases
Volume52
Issue number4
DOIs
StatePublished - 2014

Fingerprint

Erythrocyte Deformability
Sickle Cell Anemia
Heme
Oxidative Stress
Sickle Hemoglobin
Membranes
Erythrocyte Membrane
Polymerization
Erythrocytes

Keywords

  • Deformability
  • Erythrocytes
  • Fluorescence
  • Heme degradation
  • Hemoglobin
  • Sickle cell disease

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine
  • Hematology
  • Cell Biology

Cite this

New insights provided by a comparison of impaired deformability with erythrocyte oxidative stress for sickle cell disease. / Barodka, Viachaslau; Enika, Nagababu; Mohanty, Joy G.; Nyhan, Daniel; Berkowitz, Dan E; Rifkind, Joseph M.; Strouse, John.

In: Blood Cells, Molecules, and Diseases, Vol. 52, No. 4, 2014, p. 230-235.

Research output: Contribution to journalArticle

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