New HLA-A, -B, and -C locus-specific primers for PCR amplification from cDNA: Application in clinical immunology

Maria P. Bettinotti, Lejla Hadzikadic, Erin Ruppe, Gitika Dhillon, David S. Stroncek, Francesco M. Marincola

Research output: Contribution to journalArticle

Abstract

The individual cellular immune response to intracellular antigens is modeled by the highly polymorphic major histocompatibility complex (HLA) class I molecules. The epitopes presented and the T cell repertoire that recognizes them depend on the HLA constitution of the individual. Therefore, to monitor and to modify an individual's HLA class I-driven cellular immune response, it is necessary to know the HLA class I alleles of the person and the possible epitopes of the target antigen presented by those alleles. In particular, this is necessary in order to design peptide-based vaccines and immune therapies for the treatment of diseases caused by viruses, intracellular parasites or cancer, and to monitor the immune response during those treatments. We describe a new set of HLA-A, -B, and -C locus-specific primers for the polymerase chain reaction (PCR) amplification of the whole coding sequence of these genes from complementary DNA (cDNA). We describe their use for typing and for the production of a library of recombinant HLA class I genes. We discuss two downstream applications of this gene collection: production of soluble HLA molecules and discovery of new epitopes. Crown

Original languageEnglish (US)
Pages (from-to)143-148
Number of pages6
JournalJournal of Immunological Methods
Volume279
Issue number1-2
DOIs
StatePublished - Aug 2003
Externally publishedYes

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Keywords

  • Complementary deoxyribonucleic acid
  • Human leukocyte antigen
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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