New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region

Miroslava Štastná, Martin Trávníček, Karel Šlais

Research output: Contribution to journalArticle

Abstract

Newly prepared azo compounds and several commercially available indicators were investigated for their applicability as colored isoelectric point (p/) markers for isoelectric focusing (IEF) in the acidic range below pH 5. The majority of compounds described here can serve as primary standards since their p/ values were determined by UV-VIS spectrophotometry independently IEF and direct measurement with a pH electrode. Subjected to gel IEF they show narrow and well-observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of p/ markers covering the pH range from 1.5 to 4.7.

Original languageEnglish (US)
Pages (from-to)53-59
Number of pages7
JournalElectrophoresis
Volume26
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

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Keywords

  • Ampholytic dyes
  • p/ markers

ASJC Scopus subject areas

  • Clinical Biochemistry

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