TY - JOUR
T1 - New approach to intracardiac hemodynamic measurements in small animals
T2 - Echo-guided percutaneous apical puncture
AU - Eskesen, Kristian
AU - Olsen, Niels T.
AU - Dimaano, Veronica L.
AU - Pinheiro, Aurelio
AU - Sogaard, Peter
AU - Fritz-Hansen, Thomas
AU - Sorensen, Lars L.
AU - Abraham, Theodore P.
PY - 2012/8/1
Y1 - 2012/8/1
N2 - Objectives: Invasive measurements of intracardiac hemodynamics in animal models have allowed important advances in the understanding of cardiac disease. Currently they are performed either through a carotid arteriotomy or via a thoracotomy and apical insertion. Both of these techniques have disadvantages and are not conducive to repeated measurements. Therefore, the purpose of this study was to develop a new technique for measuring intracardiac hemodynamics. Methods: In 13 male rats, hemodynamic measurements were performed using a new echocardiographically guided percutaneous apical technique. An intravenous catheter was percutaneously inserted into the left ventricle (LV) in the direction of the LV long axis. Through this catheter, a micromanometer-tipped pressure catheter was inserted, and invasive hemodynamic traces were recorded. After LV recordings, the pressure catheter was advanced into the aorta where pressures were obtained. In 11 rats, measurements were repeated after 1 week (n = 2), 2 weeks (n = 4), 3 weeks (n = 4), or 4 weeks (n = 1). In 3 rats, invasive measurements were performed using a carotid arteriotomy before the percutaneous technique. Results: Among the 13 rats subjected to the procedure, the survival rate was 85%. Of the 11 rats that had the procedure repeated, 3 died (27%). The mean differences ± SD when comparing the two techniques were 10 ±4 mm Hg for the LV end-systolic pressure and 1 ±1 mm Hg for the LV end-diastolic pressure. The mean procedure times were 21 ±3 and 6 ±1 minutes for the carotid and percutaneous techniques, respectively. Conclusions: We have successfully developed a percutaneous technique for insertion of LV microtip catheters in rats.
AB - Objectives: Invasive measurements of intracardiac hemodynamics in animal models have allowed important advances in the understanding of cardiac disease. Currently they are performed either through a carotid arteriotomy or via a thoracotomy and apical insertion. Both of these techniques have disadvantages and are not conducive to repeated measurements. Therefore, the purpose of this study was to develop a new technique for measuring intracardiac hemodynamics. Methods: In 13 male rats, hemodynamic measurements were performed using a new echocardiographically guided percutaneous apical technique. An intravenous catheter was percutaneously inserted into the left ventricle (LV) in the direction of the LV long axis. Through this catheter, a micromanometer-tipped pressure catheter was inserted, and invasive hemodynamic traces were recorded. After LV recordings, the pressure catheter was advanced into the aorta where pressures were obtained. In 11 rats, measurements were repeated after 1 week (n = 2), 2 weeks (n = 4), 3 weeks (n = 4), or 4 weeks (n = 1). In 3 rats, invasive measurements were performed using a carotid arteriotomy before the percutaneous technique. Results: Among the 13 rats subjected to the procedure, the survival rate was 85%. Of the 11 rats that had the procedure repeated, 3 died (27%). The mean differences ± SD when comparing the two techniques were 10 ±4 mm Hg for the LV end-systolic pressure and 1 ±1 mm Hg for the LV end-diastolic pressure. The mean procedure times were 21 ±3 and 6 ±1 minutes for the carotid and percutaneous techniques, respectively. Conclusions: We have successfully developed a percutaneous technique for insertion of LV microtip catheters in rats.
KW - Cardiac instrumentation
KW - Experimental models
KW - Invasive hemodynamic measurements
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U2 - 10.7863/jum.2012.31.8.1233
DO - 10.7863/jum.2012.31.8.1233
M3 - Article
C2 - 22837287
AN - SCOPUS:84864648765
SN - 0278-4297
VL - 31
SP - 1233
EP - 1238
JO - Journal of Ultrasound in Medicine
JF - Journal of Ultrasound in Medicine
IS - 8
ER -