Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability

Bryan F. Shaw, Haribabu Arthanari, Max Narovlyansky, Armando Durazo, Dominique P Frueh, Michael P. Pollastri, Andrew Lee, Basar Bilgicer, Steven P. Gygi, Gerhard Wagner, George M. Whitesides

Research output: Contribution to journalArticle

Abstract

This paper combines two techniques-mass spectrometry and protein charge ladders-to examine the relationship between the surface charge and hydrophobicity of a representative globular protein (bovine carbonic anhydrase II; BCA II) and its rate of amide hydrogen-deuterium (H/D) exchange. Mass spectrometric analysis indicated that the sequential acetylation of surface lysine-ε-NH3+ groups-a type of modification that increases the net negative charge and hydrophobicity of the surface of BCA II without affecting its secondary or tertiary structure-resulted in a linear decrease in the aggregate rate of amide H/D exchange at pD 7.4, 15 °C. According to analysis with MS, the acetylation of each additional lysine generated between 1.4 and 0.9 additional hydrogens that are protected from H/D exchange during the 2 h exchange experiment at 15 °C, pD 7.4. NMR spectroscopy demonstrated that none of the hydrogen atoms which became protected upon acetylation were located on the side chain of the acetylated lysine residues (i.e., lys-ε-NHCOCH3) but were instead located on amide NHCO moieties in the backbone. The decrease in rate of exchange associated with acetylation paralleled a decrease in thermostability: the most slowly exchanging rungs of the charge ladder were the least thermostable (as measured by differential scanning calorimetry). This observation-that faster rates of exchange are associated with slower rates of denaturation-is contrary to the usual assumptions in protein chemistry. The fact that the rates of H/D exchange were similar for perbutyrated BCA II (e.g., [lys-ε-NHCO(CH 2)2CH3]18) and peracetylated BCA II (e.g., [lys-ε-NHCOCH3]18) suggests that the electrostatic charge is more important than the hydrophobicity of surface groups in determining the rate of H/D exchange. These electrostatic effects on the kinetics of H/D exchange could complicate (or aid) the interpretation of experiments in which H/D exchange methods are used to probe the structural effects of non-isoelectric perturbations to proteins (i.e., phosphorylation, acetylation, or the binding of the protein to an oligonucleotide or to another charged ligand or protein).

Original languageEnglish (US)
Pages (from-to)17411-17425
Number of pages15
JournalJournal of the American Chemical Society
Volume132
Issue number49
DOIs
StatePublished - Dec 15 2010
Externally publishedYes

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Amides
Deuterium
Hydrogen
Membrane Proteins
Acetylation
Proteins
Hydrophobicity
Hydrophobic and Hydrophilic Interactions
Lysine
Ladders
Static Electricity
Electrostatics
Carbonic anhydrase
Carbonic Anhydrase II
Phosphorylation
Denaturation
Oligonucleotides
Differential Scanning Calorimetry
Surface charge
Nuclear magnetic resonance spectroscopy

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability. / Shaw, Bryan F.; Arthanari, Haribabu; Narovlyansky, Max; Durazo, Armando; Frueh, Dominique P; Pollastri, Michael P.; Lee, Andrew; Bilgicer, Basar; Gygi, Steven P.; Wagner, Gerhard; Whitesides, George M.

In: Journal of the American Chemical Society, Vol. 132, No. 49, 15.12.2010, p. 17411-17425.

Research output: Contribution to journalArticle

Shaw, BF, Arthanari, H, Narovlyansky, M, Durazo, A, Frueh, DP, Pollastri, MP, Lee, A, Bilgicer, B, Gygi, SP, Wagner, G & Whitesides, GM 2010, 'Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability', Journal of the American Chemical Society, vol. 132, no. 49, pp. 17411-17425. https://doi.org/10.1021/ja9067035
Shaw, Bryan F. ; Arthanari, Haribabu ; Narovlyansky, Max ; Durazo, Armando ; Frueh, Dominique P ; Pollastri, Michael P. ; Lee, Andrew ; Bilgicer, Basar ; Gygi, Steven P. ; Wagner, Gerhard ; Whitesides, George M. / Neutralizing positive charges at the surface of a protein lowers its rate of amide hydrogen exchange without altering its structure or increasing its thermostability. In: Journal of the American Chemical Society. 2010 ; Vol. 132, No. 49. pp. 17411-17425.
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