Neutralizing and fusion-blocking antibodies induced by recombinant visna virus envelope glycoprotein

David L. Huso

Research output: Contribution to journalArticle

Abstract

A recombinant envelope glycoprotein derived from visna virus, a natural lentivirus pathogen of sheep, induced antibodies that neutralized cell-free virus and blocked virus-mediated cell-to-cell fusion. The visna virus envelope gene was subcloned into a baculovirus expression vector and was expressed in insect cells. A pair of guinea pigs were immunized with the recombinant glycoprotein, and postimmunization sera neutralized cell-free visna virus infectivity and also blocked virus-mediated syncytium formation when infected and uninfected cells were cocultured together. Thus, the recombinant visna surface envelope glycoprotein is capable of inducing both neutralizing and fusion-blocking antibodies. In addition to its relevance for vaccine development, the recombinant glycoprotein will be a useful tool to study differences between antibody-mediated enhancement versus neutralization during virus-host interactions in lentivirus infections in vivo.

Original languageEnglish (US)
Pages (from-to)15-20
Number of pages6
JournalViral Immunology
Volume10
Issue number1
StatePublished - 1997

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Visna-maedi virus
Blocking Antibodies
Glycoproteins
Viruses
Visna
Lentivirus Infections
Lentivirus
Synthetic Vaccines
Cell Fusion
Antibodies
Baculoviridae
Membrane Glycoproteins
Giant Cells
Insects
Sheep
Guinea Pigs
Serum
Genes

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Neutralizing and fusion-blocking antibodies induced by recombinant visna virus envelope glycoprotein. / Huso, David L.

In: Viral Immunology, Vol. 10, No. 1, 1997, p. 15-20.

Research output: Contribution to journalArticle

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