Neutralization of bovine papillomavirus by antibodies to L1 and L2 capsid proteins

Richard B.S. Roden, Eva M. Weissinger, Darren W. Henderson, Frank Booy, Reinhard Kirnbauer, J. Frederic Mushinski, Douglas R. Lowy, John T. Schiller

Research output: Contribution to journalComment/debatepeer-review


We have generated four mouse monoclonal antibodies (MAbs) to bovine papillomavirus virions that bound type-specific, adjacent, and conformationally dependent epitopes on the L1 major capsid protein. All four MAbs were neutralizing at ratios of 1 MAb molecule per 5 to 25 L1 molecules, but only three effectively blocked binding of the virus to the cell surface. Therefore, antibodies can prevent papillomavirus infection by at least two mechanisms: inhibition of cell surface receptor binding and a subsequent step in the infectious pathway. The neutralizing epitopes of the bovine papillomavirus L2 minor capsid protein were mapped to the N-terminal half of L2 by blocking the neutralizing activity of full-length L2 antiserum with bacterially expressed peptides of L2. In addition, rabbit antiserum raised against amino acids 45 to 173 of L2 had a neutralizing titer of 1,000, confirming that at least part of the N terminus of L2 is exposed on the virion surface.

Original languageEnglish (US)
Pages (from-to)7570-7574
Number of pages5
JournalJournal of virology
Issue number11
StatePublished - Nov 1994
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology


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