Neurovascular coupling: In vivo optical techniques for functional brain imaging

Lun De Liao, Vassiliy Tsytsarev, Ignacio Delgado-Martínez, Meng Lin Li, Reha Erzurumlu, Ashwati Vipin, Josue Orellana, Yan Ren Lin, Hsin Yi Lai, You Yin Chen, Nitish V Thakor

Research output: Contribution to journalArticle

Abstract

Optical imaging techniques reflect different biochemical processes in the brain, which is closely related with neural activity. Scientists and clinicians employ a variety of optical imaging technologies to visualize and study the relationship between neurons, glial cells and blood vessels. In this paper, we present an overview of the current optical approaches used for the in vivo imaging of neurovascular coupling events in small animal models. These techniques include 2-photon microscopy, laser speckle contrast imaging (LSCI), voltage-sensitive dye imaging (VSDi), functional photoacoustic microscopy (fPAM), functional near-infrared spectroscopy imaging (fNIRS) and multimodal imaging techniques. The basic principles of each technique are described in detail, followed by examples of current applications from cutting-edge studies of cerebral neurovascular coupling functions and metabolic. Moreover, we provide a glimpse of the possible ways in which these techniques might be translated to human studies for clinical investigations of pathophysiology and disease. In vivo optical imaging techniques continue to expand and evolve, allowing us to discover fundamental basis of neurovascular coupling roles in cerebral physiology and pathophysiology.

Original languageEnglish (US)
Article number38
JournalBioMedical Engineering Online
Volume12
Issue number1
DOIs
StatePublished - Apr 30 2013

Fingerprint

Functional Neuroimaging
Optical Imaging
Brain
Imaging techniques
Voltage-Sensitive Dye Imaging
Biochemical Phenomena
Multimodal Imaging
Near-Infrared Spectroscopy
Photons
Confocal Microscopy
Neuroglia
Blood Vessels
Microscopy
Animal Models
Technology
Neurons
Photoacoustic microscopy
Near infrared spectroscopy
Neurovascular Coupling
Physiology

Keywords

  • 2-photon microscopy
  • Cerebral neuroimaging
  • Functional near-infrared spectroscopy
  • Functional photoacoustic microscopy
  • Laser speckle contrast imaging
  • Neurovascular coupling
  • Voltage sensitive dye imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging
  • Biomaterials

Cite this

Liao, L. D., Tsytsarev, V., Delgado-Martínez, I., Li, M. L., Erzurumlu, R., Vipin, A., ... Thakor, N. V. (2013). Neurovascular coupling: In vivo optical techniques for functional brain imaging. BioMedical Engineering Online, 12(1), [38]. https://doi.org/10.1186/1475-925X-12-38

Neurovascular coupling : In vivo optical techniques for functional brain imaging. / Liao, Lun De; Tsytsarev, Vassiliy; Delgado-Martínez, Ignacio; Li, Meng Lin; Erzurumlu, Reha; Vipin, Ashwati; Orellana, Josue; Lin, Yan Ren; Lai, Hsin Yi; Chen, You Yin; Thakor, Nitish V.

In: BioMedical Engineering Online, Vol. 12, No. 1, 38, 30.04.2013.

Research output: Contribution to journalArticle

Liao, LD, Tsytsarev, V, Delgado-Martínez, I, Li, ML, Erzurumlu, R, Vipin, A, Orellana, J, Lin, YR, Lai, HY, Chen, YY & Thakor, NV 2013, 'Neurovascular coupling: In vivo optical techniques for functional brain imaging', BioMedical Engineering Online, vol. 12, no. 1, 38. https://doi.org/10.1186/1475-925X-12-38
Liao LD, Tsytsarev V, Delgado-Martínez I, Li ML, Erzurumlu R, Vipin A et al. Neurovascular coupling: In vivo optical techniques for functional brain imaging. BioMedical Engineering Online. 2013 Apr 30;12(1). 38. https://doi.org/10.1186/1475-925X-12-38
Liao, Lun De ; Tsytsarev, Vassiliy ; Delgado-Martínez, Ignacio ; Li, Meng Lin ; Erzurumlu, Reha ; Vipin, Ashwati ; Orellana, Josue ; Lin, Yan Ren ; Lai, Hsin Yi ; Chen, You Yin ; Thakor, Nitish V. / Neurovascular coupling : In vivo optical techniques for functional brain imaging. In: BioMedical Engineering Online. 2013 ; Vol. 12, No. 1.
@article{ffb360842d8743779418096df0026a7b,
title = "Neurovascular coupling: In vivo optical techniques for functional brain imaging",
abstract = "Optical imaging techniques reflect different biochemical processes in the brain, which is closely related with neural activity. Scientists and clinicians employ a variety of optical imaging technologies to visualize and study the relationship between neurons, glial cells and blood vessels. In this paper, we present an overview of the current optical approaches used for the in vivo imaging of neurovascular coupling events in small animal models. These techniques include 2-photon microscopy, laser speckle contrast imaging (LSCI), voltage-sensitive dye imaging (VSDi), functional photoacoustic microscopy (fPAM), functional near-infrared spectroscopy imaging (fNIRS) and multimodal imaging techniques. The basic principles of each technique are described in detail, followed by examples of current applications from cutting-edge studies of cerebral neurovascular coupling functions and metabolic. Moreover, we provide a glimpse of the possible ways in which these techniques might be translated to human studies for clinical investigations of pathophysiology and disease. In vivo optical imaging techniques continue to expand and evolve, allowing us to discover fundamental basis of neurovascular coupling roles in cerebral physiology and pathophysiology.",
keywords = "2-photon microscopy, Cerebral neuroimaging, Functional near-infrared spectroscopy, Functional photoacoustic microscopy, Laser speckle contrast imaging, Neurovascular coupling, Voltage sensitive dye imaging",
author = "Liao, {Lun De} and Vassiliy Tsytsarev and Ignacio Delgado-Mart{\'i}nez and Li, {Meng Lin} and Reha Erzurumlu and Ashwati Vipin and Josue Orellana and Lin, {Yan Ren} and Lai, {Hsin Yi} and Chen, {You Yin} and Thakor, {Nitish V}",
year = "2013",
month = "4",
day = "30",
doi = "10.1186/1475-925X-12-38",
language = "English (US)",
volume = "12",
journal = "BioMedical Engineering Online",
issn = "1475-925X",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Neurovascular coupling

T2 - In vivo optical techniques for functional brain imaging

AU - Liao, Lun De

AU - Tsytsarev, Vassiliy

AU - Delgado-Martínez, Ignacio

AU - Li, Meng Lin

AU - Erzurumlu, Reha

AU - Vipin, Ashwati

AU - Orellana, Josue

AU - Lin, Yan Ren

AU - Lai, Hsin Yi

AU - Chen, You Yin

AU - Thakor, Nitish V

PY - 2013/4/30

Y1 - 2013/4/30

N2 - Optical imaging techniques reflect different biochemical processes in the brain, which is closely related with neural activity. Scientists and clinicians employ a variety of optical imaging technologies to visualize and study the relationship between neurons, glial cells and blood vessels. In this paper, we present an overview of the current optical approaches used for the in vivo imaging of neurovascular coupling events in small animal models. These techniques include 2-photon microscopy, laser speckle contrast imaging (LSCI), voltage-sensitive dye imaging (VSDi), functional photoacoustic microscopy (fPAM), functional near-infrared spectroscopy imaging (fNIRS) and multimodal imaging techniques. The basic principles of each technique are described in detail, followed by examples of current applications from cutting-edge studies of cerebral neurovascular coupling functions and metabolic. Moreover, we provide a glimpse of the possible ways in which these techniques might be translated to human studies for clinical investigations of pathophysiology and disease. In vivo optical imaging techniques continue to expand and evolve, allowing us to discover fundamental basis of neurovascular coupling roles in cerebral physiology and pathophysiology.

AB - Optical imaging techniques reflect different biochemical processes in the brain, which is closely related with neural activity. Scientists and clinicians employ a variety of optical imaging technologies to visualize and study the relationship between neurons, glial cells and blood vessels. In this paper, we present an overview of the current optical approaches used for the in vivo imaging of neurovascular coupling events in small animal models. These techniques include 2-photon microscopy, laser speckle contrast imaging (LSCI), voltage-sensitive dye imaging (VSDi), functional photoacoustic microscopy (fPAM), functional near-infrared spectroscopy imaging (fNIRS) and multimodal imaging techniques. The basic principles of each technique are described in detail, followed by examples of current applications from cutting-edge studies of cerebral neurovascular coupling functions and metabolic. Moreover, we provide a glimpse of the possible ways in which these techniques might be translated to human studies for clinical investigations of pathophysiology and disease. In vivo optical imaging techniques continue to expand and evolve, allowing us to discover fundamental basis of neurovascular coupling roles in cerebral physiology and pathophysiology.

KW - 2-photon microscopy

KW - Cerebral neuroimaging

KW - Functional near-infrared spectroscopy

KW - Functional photoacoustic microscopy

KW - Laser speckle contrast imaging

KW - Neurovascular coupling

KW - Voltage sensitive dye imaging

UR - http://www.scopus.com/inward/record.url?scp=84876811672&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84876811672&partnerID=8YFLogxK

U2 - 10.1186/1475-925X-12-38

DO - 10.1186/1475-925X-12-38

M3 - Article

C2 - 23631798

AN - SCOPUS:84876811672

VL - 12

JO - BioMedical Engineering Online

JF - BioMedical Engineering Online

SN - 1475-925X

IS - 1

M1 - 38

ER -